摘要
目的探讨微小RNA-146a-5p(miR-146a-5p)与微小RNA-21(miR-21)交互作用对原发性肾病综合征(primary nephrotic syndrome,NS)发病风险及病情程度的关系。方法选取2021年8月至2023年8月NS患者103例作为研究组,另选取同期体检的健康志愿者103名作为对照组。比较研究组与对照组、研究组不同病理类型[微小病变型(minimal change disease,MCD)、膜性肾病(membranous nephropathy,MN)、局灶-节段性肾小球硬化症(focal segmental glomerulosclerosis,FSGS)]、研究组不同肾脏损伤程度患者的血清miR-146a-5p、miR-21相对表达量,分析miR-146a-5p、miR-21对NS的诊断价值及交互影响,进一步分析miR-146a-5p、miR-21与病情程度的相关性。结果研究组血清miR-146a-5p相对表达量低于对照组,miR-21相对表达量高于对照组(P<0.05);研究组不同病理类型患者血清miR-146a-5p、miR-21相对表达量比较,差异有统计学意义(P<0.05);研究组不同肾脏损伤程度患者血清miR-146a-5p、miR-21相对表达量比较,差异有统计学意义(P<0.05);miR-146a-5p、miR-21联合诊断NS的AUC为0.923(95%CI:0.877~0.955),敏感度为78.64%,特异度为90.29%,明显优于miR-146a-5p、miR-21单独诊断;以最佳截断值将miR-146a-5p、miR-21分为高表达与低表达,miR-146a-5p低表达与miR-21高表达在NS发病风险呈正向交互作用,为次相乘模型;miR-146a-5p与NS病理类型、肾脏损伤程度呈负相关(r=-0.613、-0.657,P<0.05),miR-21与NS病理类型、肾脏损伤程度呈正相关(r=0.664、0.702,P<0.05)。结论NS患者血清中miR-146a-5p、miR-21异常表达,且与患者病理类型、肾脏损伤程度有关;此外,miR-146a-5p、miR-21在NS发病风险中具有正向交互作用,同时暴露可增加NS发病风险。
Objective To investigate the relationship between the interaction of microRNA-146a-5p(miR-146a-5p)and microRNA-21(miR-21)and its effect on the risk and severity of primary nephrotic syndrome(NS).Methods A total of 103 patients with NS from August 2021 to August 2023 were selected as the study group,and another 103 healthy volunteers who underwent physical examination during the same period were selected as the control group.Compare the different pathological types[minimal change disease(MCD),membranous nephropathy(MN),focal segmental glomerulosclerosis(FSGS)]and the degree of renal injury in the two groups,and study the relative expression levels of serum miR-146a-5p and miR-21 in patients.Analyze the diagnostic value and interactive effects of miR-146a-5p and miR-21 on NS,and further analyze the correlation between miR-146a-5p,miR-21,and disease severity.Results The relative expression level of miR-146a-5p in the study group was lower than that in the control group,and the relative expression level of miR-21 was higher than that in the control group(P<0.05).The relative expression levels of serum miR-146a-5p and miR-21 in patients with different pathological types or different degrees of renal injury in the study group showed significant differences(both P<0.05).The AUC of the combined diagnosis of NS by miR-146a-5p and miR-21 was 0.923(95%CI:0.877-0.955),the sensitivity was 78.64%,and the specificity was 90.29%,which were significantly better than those of miR-146a-5p and miR-21 alone.The optimal cut-off value was used to divide miR-146a-5p and miR-21 into high expression group and low expression group.The low expression of miR-146a-5p and high expression of miR-21 had a positive interaction on the risk of NS development,which was a sub-multiplication model.miR-146a-5p was negatively correlated with NS pathological types and the degree of kidney injury(r=-0.613,-0.657,P<0.05),while miR-21 was positively correlated with NS pathological types and the degree of kidney injury(r=0.664,0.702,P<0.05).Conclusion The ab
作者
陈景荷
张艳琴
李文冬
CHEN Jinghe;ZHANG Yanqin;LI Wendong(Department of Critical Care Medicine,Baoji People’s Hospital,Baoji,Shaanxi,721000,China;Department of Nephrology,Baoji People’s Hospital,Baoji,Shaanxi,721000,China)
出处
《医学分子生物学杂志》
CAS
2024年第6期551-556,共6页
Journal of Medical Molecular Biology
基金
陕西省自然科学基础研究计划(No.2023-JC-YB-644)。
