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中草药制剂对湖羊血清生化和免疫指标、肠道组织形态及消化酶活性的影响

Effects of Chinese Herbal Preparations on Serum Biochemical and Immune Indicators,Intestinal Tissue Morphology and Digestive Enzyme Activities in Lake Sheep
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摘要 试验旨在探究中草药制剂对湖羊血清生化和免疫指标、肠道组织形态以及消化酶活性的影响。选择平均体重为(19.60±1.63)kg的3月龄健康湖羊公羔18只,随机分为3组(对照组、A组、B组),每组6只羊。对照组饲喂基础日粮,A组和B组在精料补充料中添加0.5%和1.0%的中草药制剂,试验期90 d。结果表明:①A组总蛋白(TP)含量显著高于对照组(P<0.05),对照组的白蛋白(ALB)含量显著低于A组和B组(P<0.05)。②免疫球蛋白A(IgA)含量B组极显著高于对照组和A组(P<0.01),免疫球蛋白M(IgM)含量对照组极显著低于A组和B组(P<0.01),溶菌酶活性对照组极显著低于A组和B组(P<0.01)。③回肠绒毛高度B组极显著高于A组和对照组(P<0.01),肌层厚度对照组极显著低于A组和B组(P<0.01);十二指肠肌层厚度B组显著高于A组(P<0.05),绒隐比B组显著高于对照组(P<0.05);对照组空肠绒毛高度、绒隐比极显著低于A组和B组(P<0.01)。说明中草药制剂可显著提高湖羊机体免疫力和肠道吸收利用能力,1%的添加量效果较好。 The aim of this experiment was to inves⁃tigate the effects of Chinese herbal preparations on serum biochemical and immune indexes,intestinal tissue morphology and digestive enzyme activity of Hu sheep.Eighteen 3-month-old healthy Hu male lambs with an average body weight of(19.60±1.63)kg were randomly divided into 3 groups(control group,group A and group B)with 6 sheep in each group.The sheep in control group were fed a basal diet,and the sheep in groups A and B were fed 0.5%and 1.0%Chinese herbal preparations in concen⁃trate supplement.The experiment lasted for 90 days.The results showed as follows:①the total protein(TP)of sheep in group A was significantly higher than that of in control group(P<0.05),and the albumin(ALB)of control group was extremely significantly lower than that of groups A and B(P<0.05).②Immu⁃noglobulin A(IgA)in group B was extremely significantly higher than that of in control group and group A(P<0.01),immunoglobulin M(IgM)in control group was extremely significantly lower than that of groups A and B(P<0.01),lysozyme activity in control group was extremely significantly lower than that of groups A and B(P<0.01).③Ileum villi length in group B was extremely significantly higher than that in group A and control group(P<0.01),and muscle thickness in control group was extremely significantly lower than that in groups A and B(P<0.01).The muscle thickness of duodenum group B was significantly higher than that of group A(P<0.05),and the ratio of velvet to crypto in group B was significantly higher than that of control group(P<0.05).The villus length and pile-to-crypto ratio of jejunum in control group were extremely significantly lower than those in groups A and B(P<0.01).The results showed that Chi⁃nese herbal preparations could significantly improve the immunity and intestinal absorption and utiliza⁃tion ability of Hu sheep,the effect of 1%addition is better.
作者 李婉宁 李乔 齐兴财 栗登攀 王春卉 刘子龙 李讨讨 马友记 LI Wanning;LI Qiao;QI Xingcai;LI Dengpan;WANG Chunhui;LIU Zilong;LI Taotao;MA Youji(College of Animal Science and Technology,Gansu Agricultural University,Gansu Lanzhou 730070,China)
出处 《饲料工业》 CAS 北大核心 2024年第21期100-105,共6页 Feed Industry
基金 临夏州牛羊产业重大科技支撑项目[KJJC-LX-2023-05] 甘肃农业大学学科团队项目[GAU-XKTD-2022-20]。
关键词 中草药制剂 血清生化 血清免疫 肠道 组织形态 消化酶 herbal preparations serum biochemistry serum immunity intestinal tract tissue morphology digestive enzymes
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