摘要
目的对不同基原淫羊藿综合品质进行研究,为其质量评价及开发利用提供依据。方法分析采用Agilent ZORBAX SB-C _(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长270 nm。建立29批样品HPLC指纹图谱。采用聚类分析、主成分分析、正交偏最小二乘法-判别分析进行化学模式识别。结果29批样品指纹图谱中有12个共有峰,指认出其中5个,相似度0.235~0.999。不同基原淫羊藿中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ含量有明显差异。各批样品聚为4类,3号峰、朝藿定B、朝藿定C、淫羊藿苷为主要成分。结论该方法专属性强,稳定可靠,可快速鉴别不同基原淫羊藿,并能对其进行质量评价。
AIM To study the comprehensive quality of Epimedii Folium from different botanical origins,and to provide a basis for its quality assessment to ensure its further development and utilization.METHODS The analysis was performed on a 30℃thermostatic Agilent ZORBAX SB-C_(18) column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile -0.1% phosphate flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 270 nm.With regard to the established HPLC fingerprints,the twenty-nine batches of samples achieved their chemical pattern recognition by using cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis.RESULTS Among the twelve common peaks in the fingerprint of twenty-nine batches of samples,five were identified,sharing a similarity within the range of 0.235-0.999.Displaying significant content differences in epimedin A,epimedin B,epimedin C,icariin,and pogostemin I.Each batch of E.Folium of different origins categorized into 4 types was observed with peak 3,epimedin B,epimedin C,and icariin as the main components.CONCLUSION This method of strong specificity,stability and reliability can be applicable in the quick identification of E.Folium from different botanical origins and its quality assessment.
作者
翟英茹
罗江南
李慧
黄璐琦
ZHAI Ying-ru;LUO Jiang-nan;LI Hui;HUANG Lu-qi(Academician Workstation of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Institute of Traditional Chinese Medicine Health Industry,China Academy of Chinese Medical Sciences,Nanchang 330029,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第10期3197-3203,共7页
Chinese Traditional Patent Medicine
基金
中央本级重大增减支项目(2060302)
中国中医科学院科技创新工程项目(CI2021A05205)。
关键词
淫羊藿
基原
HPLC指纹图谱
聚类分析
主成分分析
正交偏最小二乘法-判别分析
Epimedii Folium
botanical origins
HPLC fingerprints
cluster analysis
principal component analysis
orthogonal partial least squares-discriminant analysis
