摘要
活性分子与细胞膜之间的相互作用在许多基本的生物过程中扮演着至关重要的角色,然而如何实现对此界面动力学过程的原位、实时、无标记且无侵入监测仍是生物物理研究领域所面临的一大挑战.我们与合作者开发的光电压瞬态技术,为解决这一问题提供了一种新途径.该技术利用硅片光电响应生成电荷,并将磷脂膜的充放电过程记录为电压瞬态脉冲、建立了该充放电过程与界面瞬时结构和性质之间的关联性.因此,通过对随时间演化的电压脉冲进行分析,可以揭示活性分子作用下膜结构实时动态变化情况,尤其是不同作用状态之间转换的时间信息,可作为传统技术的有益补充.同时,该技术设备搭建成本低廉,操作方便,无需复杂的数据处理过程.本综述概述了光电压瞬态技术的工作原理、设备搭建以及数据处理方法,并以经典细胞膜模型——磷脂双层膜为例,总结了该技术在探索磷脂膜水合特性及其与活性分子(如表面活性剂、聚合物、多肽和纳米颗粒)相互作用机制方面取得的最新进展.最后就该技术优缺点进行讨论并展望未来发展前景.
The dynamic interactions between active molecules and the cell membrane play a crucial role in various fundamental biological processes.The photovoltage transient technique developed in recent years has provided an in situ,real-time,and non-invasive approach to studying dynamic processes at the membrane interface.In this technique,the photoelectric response of silicon wafers is used to generate charges and record voltage transient pulses in the charging and discharging process of phospholipid membranes.These pulses directly reflect the instantaneous structure and properties of the membrane.By analyzing the temporal evolution of voltage pulses,the dynamic changes in membrane structure induced by molecular actions can be elucidated.In particular,this technique provides valuable insights into the timing of transitions between different functional states.In this paper,the working principle,equipment setup,and data processing methods used in photovoltage transient analysis are comprehensively reviewed.Furthermore,the use of supported phospholipid bilayers as model cell membranes highlights recent progress of this technique in investigating the mechanisms underlying membrane interactions of active molecules such as surfactants,polymers,peptides,and nanoparticles.Finally,its advantages and limitations are evaluated,and its development prospects are discussed.The photovoltage transient technique is initially used to analyze the charging and discharging curves,as well as the hydration process,of single-and multi-layered membranes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC)phospholipids.Previously,X-ray diffraction(XRD)and quartz crystal microbalance with dissipation(QCM-D)technology were commonly utilized for the real-time monitoring of the swelling process in phospholipid membranes,providing the information about changes in mass and thickness of Z-direction layers.In contrast,the photovoltage transient technique presents additional insights into the kinetics of the swelling process and timing of transition
作者
窦玉江
李文文
魏裕双
杨恺
元冰
Dou Yu-Jiang;Li Wen-Wen;Wei Yu-Shuang;Yang Kai;Yuan Bing(School of Electronic Information,Dongguan Polytechnic,Dongguan 523808,China;Songshan Lake Materials Laboratory,Dongguan 523808,China;Center for Soft Condensed Matter Physics and Interdisciplinary Research&School of Physical Science and Technology,Soochow University,Suzhou 215006,China)
出处
《物理学报》
SCIE
EI
CAS
CSCD
北大核心
2024年第21期22-36,共15页
Acta Physica Sinica
基金
国家自然科学基金(批准号:12274307,32230063,12347102)
广东省基础与应用基础研究基金(批准号:2023A1515011610)
广东省教育厅2023年度特色创新项目(自然科学)(批准号:2023KTSCX357)
东莞职业技术学院科研创新基金项目(批准号:KYCX202410)资助的课题。
