摘要
黄花蒿(Artemisia annua)提取物青蒿琥酯(Artesunate)是一种具有多种药理活性的天然化合物,广泛用于治疗疟疾及多种炎症性疾病。本研究旨在探讨青蒿琥酯对运动性心肌损伤大鼠心肌铁离子含量和铁死亡相关蛋白表达的影响。通过建立运动性心肌损伤动物模型,分别检测了大鼠心肌中的ROS、MDA、GSH、Fe2+含量及铁死亡相关蛋白(GPX4,FTH1,FPN1)的表达水平。结果显示,青蒿琥酯显著改善了运动性心肌损伤大鼠的心功能,降低了心肌损伤标志物(cTnI,CK-MB,LDH)的水平,减少了心肌细胞凋亡。此外,青蒿琥酯显著降低了心肌中的ROS和MDA水平,提升了GSH水平,抑制了氧化应激。青蒿琥酯还通过减少心肌Fe2+含量和上调GPX4、FTH1、FPN1蛋白的表达,减轻了铁死亡。综上所述,青蒿琥酯通过调控铁代谢和抑制铁死亡,对运动性心肌损伤大鼠具有显著的保护作用,具有潜在的应用价值。
Artemisia annua extract,artesunate,is a natural compound with various pharmacological activities,widely used in the treatment of malaria and various inflammatory diseases.This study aims to investigate the effects of artesunate on myocardial iron ion content and ferroptosis-related protein expression in rats with exercise-induced myocardial injury.By establishing an animal model of exercise-induced myocardial injury,we measured the levels of ROS,MDA,GSH,Fe^(2+),and the expression levels of ferroptosis-related proteins(GPX4,FTH1,FPN1)in rat myocardium.The results showed that artesunate significantly improved the cardiac function of rats with exercise-induced myocardial injury,reduced the levels of myocardial injury markers(c TnI,CK-MB,LDH),and decreased myocardial cell apoptosis.Additionally,artesunate significantly reduced the levels of ROS and MDA in the myocardium,increased GSH levels,and inhibited oxidative stress.Artesunate also alleviated ferroptosis by reducing myocardial Fe^(2+) content and upregulating the expression of GPX4,FTH1,and FPN1 proteins.In conclusion,artesunate exerts significant protective effects on rats with exercise-induced myocardial injury by regulating iron metabolism and inhibiting ferroptosis,indicating its potential application value.
作者
吕鹏飞
LüPengfei(Zhengzhou Sias College,Xinzheng,450044)
出处
《分子植物育种》
CAS
北大核心
2024年第21期7222-7229,共8页
Molecular Plant Breeding
关键词
黄花蒿
青蒿琥酯
动性心肌损伤(EIMI)
铁代谢
铁死亡
Artemisia annua,Artesunate
Exercise-induced myocardial injury(EIMD)
Iron metabolism
Ferroptosis
