摘要
为了解AP2/ERF转录因子家族的euANT亚家族成员Zm AIL1在玉米生长发育中的作用,本研究以玉米‘先玉335’为试验材料,采用RT-PCR技术克隆了玉米AP2亚家族成员Zm AIL1基因的全长cDNA,并对其进行了生物信息学分析,通过实时定量PCR检测了其在不同组织和不同胁迫条件下的表达模式。结果表明,ZmAIL1基因的全长cDNA为1273 bp,含有一个873 bp的开放阅读框,可编码290个氨基酸,相对分子质量为27.258 kD,理论等电点为8.69,为疏水性蛋白。系统进化分析表明,该基因与高粱(Sorghum bicolor L.)等禾本科植物具有较近的亲缘关系。RT-qPCR分析表明它主要在旗叶和花丝中表达,在受到不同非生物胁迫条件下均能表达,在盐和干旱胁迫后的幼苗叶片组织中显著上调表达。该研究结果为深入探究ZmAIL1基因功能提供了科学依据。
To understand the role of ZmAIL1,a member of the euANT subfamily of the AP2/ERF transcription factor family,in maize growth and development,the full-length cDNA of Zm AIL1 gene was cloned using RT-PCR with maize'Xianyu335'as the experimental material.Bioinformatics analysis was conducted,and its expression pattern in different tissues and under abiotic stress conditions was examined by real-time quantitative PCR.The results showed that the cDNA length of Zm AIL1 was 1273 bp,containing an 873 bp open reading frame and encoding 290 amino acids,with a relative molecular weight is 27.258 kD and a theoretical isoelectric point of 8.69,and was a hydrophobic protein.Phylogenetic analysis showed that it was closely related to Sorghum bicolor and other gramineous plants.RT-qPCR analysis showed that it was mainly expressed in leaves and tassels,and the expression was induced under different abiotic stress conditions.Moreover,the expression levels of Zm AIL1 were significantly up-regulated in seedling leaf tissues under salt and drought stress.These results provide a scientific basis for further exploring the function of Zm AIL1 gene.
作者
郭海滨
郝立冬
Guo Haibin;Hao Lidong(College of Agriculture and Hydraulic Engineering,Suihua University,Suihua,152000)
出处
《分子植物育种》
CAS
北大核心
2024年第21期6980-6985,共6页
Molecular Plant Breeding
基金
黑龙江省省属高等学校基本科研业务费优秀创新团队建设项目(KYYWF10236190302)资助。
