摘要
目的:探讨PUM1基因对胶质瘤细胞增殖、迁移及侵袭能力的影响。方法:siRNA干扰PUM1在胶质瘤U373和U251细胞中表达;RT-PCR检测siRNA-PUM1干扰前后PUM1在U373和U251细胞中表达;CCK-8评估siRNA-PUM1干扰前后U373和U251细胞增殖能力;TUNEL评估siRNA-PUM1干扰前后U373和U251细胞凋亡;细胞划痕评估siRNA-PUM1干扰前后U373和U251细胞迁移能力;Transwell评估siRNA-PUM1干扰前后U373和U251细胞侵袭能力;乳酸试剂盒检测siRNA-PUM1干扰前后U373和U251细胞乳酸表达变化;Western blotting检测siRNA-PUM1干扰前后U373和U251细胞PKM2和Parkin的表达变化。结果:siRNA-PUM1显著降低PUM1在U373和U251细胞中表达;siRNA-PUM1显著抑制U373和U251细胞增殖、迁移和侵袭能力;siRNA-PUM1显著抑制U373和U251细胞糖酵解以及PKM2和Parkin表达。结论:siRNA干扰PUM1基因可能通过抑制糖酵解途径抑制胶质瘤U373和U251细胞增殖、迁移及侵袭能力。
Objective:To explore the effect of PUM1 gene on proliferation,migration and invasion ability of glioma cells.Methods:siRNA interfered with the expression of PUM1 in U373 and U251 cells.The expressions of PUM1 mRNA in U373 and U251 cell lines were detected using RT-qPCR.CCK-8 assay was employed to assess the proliferative capacity of U373 and U251 cells pre-and post-siRNA-mediated interference of PUM1.The apoptosis of U373 and U251 cells was assessed by TUNEL assay before and after interference with siRNA-PUM1.Cell scratch assay was used to evaluate the migration ability of U373 and U251 cells before and after PUM1 siRNA interference.The invasion ability of U373 and U251 cells was assessed using Transwell before and after interference with siRNA-PUM1.The lactate assay kit was utilized to assess the alterations in lactate expression pre-and post-PUM1 silencing in U373 and U251 cells using siRNA.Western blotting detected the expression changes of PKM2 and Parkin in U373 and U251 cells before and after siRNA-PUM1 interference.Results:siRNA-PUM1 significantly decreased the expression of PUM1 in U373 and U251 cells.siRNA-PUM1 significantly suppresses the proliferation,migration,and invasive capacity of U373 and U251 cells.siRNA-PUM1 significantly suppresses glycolytic activity in U373 and U251 cells,as well as the expression of PKM2 and Parkin.Conclusion:Silencing of the PUM1 gene using siRNA may potentially impede the proliferation,migration,and invasion capabilities of U373 and U251 glioma cells by suppressing the glycolytic pathway.
作者
李京臣
孙博
葛有志
杨亮
刘晓鹏
冯岩
王苑宇
LI Jingchen;SUN Bo;GE Youzhi;YANG Liang;LIU Xiaopeng;FENG Yan;WANG Yuanyu(Neurosurgery Department,Second Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China)
出处
《现代肿瘤医学》
CAS
2024年第21期4023-4027,共5页
Journal of Modern Oncology
基金
河北省医学科学研究课题(编号:20200934)。
关键词
PUM1
胶质瘤
增殖
迁移
侵袭
糖酵解
PUM1
glioma
proliferation
migration
invasion
glycolysis
