摘要
【目的】研究纳米氧化锌(ZnO NPs)对盐碱胁迫下枸杞幼苗的生理效应,评估其缓解盐碱胁迫的生理机制及其应用潜力、适宜浓度。【方法】用1年生‘宁杞10号’枸杞盆栽幼苗为材料,先根部浇灌不同浓度(50,100,150 mg/L)ZnO NPs悬浮液,再进行100 mmol/L盐碱胁迫处理,在胁迫15 d和30 d取样测定幼苗生物量,光合色素、渗透调节物质、Na^(+)、K^(+)、活性氧(ROS)含量和抗氧化酶活性,以及AsA-GSH循环相关指标等,并综合评价处理效应。【结果】ZnO NPs预处理可以显著促进盐碱胁迫下枸杞幼苗生物量积累,增加叶片叶绿素含量,提高AsA-GSH循环抗氧化物质含量和相关酶活性以及SOD、POD、CAT活性,显著降低细胞膜损伤和脂质过氧化程度,显著提高可溶性糖、脯氨酸、可溶性蛋白积累和调节Na^(+)/K^(+)平衡,并以100 mg/L ZnO NPs处理的缓解效果最佳。【结论】根施ZnO NPs通过促进叶绿素积累,调控AsA-GSH循环和抗氧化酶活性高效清除过量ROS,提高渗透调节能力及维持Na^(+)/K^(+)平衡,有效减轻盐碱胁迫对枸杞幼苗的生长抑制。
[Objective]The study aims to investigate the effects of zinc oxide nanoparticles(ZnO NPs)on wolfberry seedlings under saline-alkali stress,evaluate its physiological mechanism for alleviating saline-alkali stress,and explore its application potential and suitable concentrations.[Methods]One-year-old‘Ningqi No.10’wolfberry seedlings in pots were used as materials.The roots were irrigated with ZnO NPs solutions of different concentrations(50,100,and 150 mg/L),followed by 100 mmol/L saline-alkali stress treatment.Samples were collected after 15 and 30 days of stress treatments to measure seedling biomass,photosynthetic pigments,osmoregulatory substances,Na^(+),K^(+),and reactive oxygen species(ROS)content,antioxidant enzyme activity,and AsA-GSH cycle-related indicators.A comprehensive evaluation of the treatments was conducted.[Results]ZnO NPs pretreatment significantly promoted the accumulation of biomass in wolfberry seedlings under saline-alkali stress,increased leaf chlorophyll con-tent,enhanced the content of antioxidant substances and related enzyme activities in the AsA-GSH cycle,as well as SOD,POD,and CAT activities.It significantly reduced cell membrane damage and lipid peroxi-dation,significantly increased the accumulation of soluble sugar,proline,and soluble protein,and regula-ted the Na^(+)/K^(+)balance.Among the tested concentrations,100 mg/L ZnO NPs treatment showed the best mitigation effect.[Conclusion]Root application of ZnO NPs effectively alleviates the growth inhibition of wolfberry seedlings under saline-alkali stress by promoting chlorophyll accumulation,regulating the AsA-GSH cycle and antioxidant enzyme activity to efficiently scavenge excessive ROS,enhancing osmotic ad-justment capacity,and maintaining Na^(+)/K^(+)balance.
作者
张荣荣
王延智
王子婷
张宝琳
ZHANG Rongrong;WANG Yanzhi;WANG Ziting;ZHANG Baolin(Baiyin Forestry and Grassland Bureau,Baiyin,Gansu 730900,China;Gansu Hengtaize Biotechnology Co.,Ltd.Baiyin,Gansu 730900,China;Gansu Academy of Forestry Sciences,Lanzhou 730020,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第11期1714-1724,共11页
Acta Botanica Boreali-Occidentalia Sinica
基金
甘肃省重点研发计划项目“陇中黄土区黑果枸杞引种及栽培利用研究”(20YF3FA010)。
