摘要
目的利用灵敏的红外传感器取代电容传感器并用于孔板嗅觉测试仪,建立小鼠嗅觉分辨、识别、空间记忆、偏好实验方法,以检验孔板嗅觉测试仪检测小鼠嗅觉功能的稳定性。方法分别采用习惯/去习惯行为测试、嗅觉空间记忆测试、丙酸嗅觉阈值测试和嗅觉偏好检测正常C57 BL/6J小鼠的嗅觉功能。结果C57 BL/6J小鼠具有嗅觉辨别、嗅觉线索关联的空间记忆能力,对丙酸的嗅觉阈值为10^(-3)kPa,对香叶醇的偏好度最高,对左旋香芹酮的偏好度最低。结论本研究使用的孔板嗅觉测试仪能够满足小鼠嗅觉的辨别、识别、学习记忆以及偏好性等嗅觉行为学实验需求。
Objective To establish experimental methods of olfactory discrimination,recognition,spatial memory and preference in mice by using sensitive infrared sensors instead of capacitive sensors and using them in the odorized hole-board test instrument in order to test the stability of the olfactory functions of mice studied by the instrument.Methods Habituation/dishabituation test,olfactory spatial memory test,propionic acid olfactory threshold test and olfactory preference test were used to evaluate the olfactory function of normal C57 BL/6J mice.Results C57 BL/6J mice were capable of olfactory discrimination and spatial memory of olfactory cue association.The olfactory threshold for propionic acid was 10^(-3) kPa,and the preference for geraniol the highest,while the preference for L-carvone the lowest.Conclusion The infrared-based odorized hole-board test instrument used in this study can satisfy the requirements of olfactory behavior experiments such as discrimination,recognition,learning and memory,and preference of mice.
作者
刘彤彤
李婧
董朝玉
王灿
解俊樊
侯一平
邵玉峰
Liu Tongtong;Li Jing;Dong Chaoyu;Wang Can;Xie Junfan;Hou Yiping;Shao Yufeng(School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,China;School of Basic Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Department of Gynecology,The Third People's Hospital of Yunnan Province,Kunming 650011,China;School of Medical Imaging,Shandong Second Medical University,Weifang 261053,Shandong,China)
出处
《兰州大学学报（医学版）》
2024年第9期6-13,34,共9页
Journal of Lanzhou University（Medical Sciences）
基金
国家自然科学基金资助项目(31872770,31500853,81771426,82001396)。
关键词
嗅觉
嗅觉功能
嗅觉行为学
嗅觉测试仪
红外传感器
小鼠
olfaction
olfactory function
olfactory behavioral experiment
odorized test instrument
infrared sensors
mice
