摘要
成株期抗叶锈病基因Lr12在生产上具有良好抗性,为Lr12进行精细定位并开发可靠的分子标记,以感病材料Thatcher和含Lr12抗性基因的近等基因系RL6011为亲本杂交产生F_(1),进一步自交产生F_(2)单株和F_(2∶3)家系。在田间利用5种强毒力叶锈菌混合小种(PHTT、THKS、THTT、PHTS和PHKS)接种F_(2)单株和F_(2∶3)家系进行成株抗叶锈性鉴定和抗性遗传分析。随后利用16K液相芯片对F_(2)的10个抗病单株和10个感病单株进行基因分型,获得与Lr12紧密连锁的SNP位点,确定抗病基因所在的染色体物理区间,开发SSR分子标记并构建遗传连锁图谱。结果表明,对RL6011(Lr12)/Thatcher的3494个F_(2)单株进行抗叶锈性鉴定,抗病单株与感病单株之间的分离比符合3∶1(χ^(2)_(3∶1)=0.14;P=0.71)。对685个F_(2∶3)家系进行抗叶锈性鉴定,抗病单株、抗病杂合单株与感病单株之间的分离比符合1∶2∶1(χ^(2)_(1∶2∶1)=2.01;P=0.37),表明Lr12为显性基因且群体分离符合单基因遗传规律。通过遗传连锁图谱分析成株期抗叶锈病基因Lr12基因位于SSR分子标记YK12817和YK12928之间,遗传区间为0.38 cM,对应中国春参考基因组(IWGSC.Ref.V1.0)中4BL染色体579.44~581.53 Mb物理范围内共2.09 Mb的物理区间。上述结果为预测候选基因提供了确定的依据。
The adult plant resistance gene Lr12 exhibits excellent resistance in production systems.To fine map and develop reliable molecular markers for Lr12,a cross was made between the susceptible variety Thatcher and the resistant near-isogenic line RL6011 containing the Lr12 gene.The F_(1)generation resulting from this cross was self-pollinated to generate F_(2)individual plants and F_(2∶3)families.Field evaluations were conducted using a mixture of five highly virulent leaf rust pathotypes(PHTT,THKS,THTT,PHTS,and PHKS)to inoculate F 2 individual plants and F_(2∶3)families for adult plant resistance assessment and genetic analysis of resistance.Subsequently,genotyping was performed using a 16K liquid chip on 10 resistant and 10 susceptible individuals from the F 2 generation to identify SNP markers closely linked to Lr12.This enabled the determination of the chromosomal physical interval containing the resistance gene,the development of SSR molecular markers,and the construction of a genetic linkage map.The results indicate that the segregation ratio of resistance to leaf rust in 3494 F_(2)individuals derived from the RL6011(Lr12)/Thatcher cross was consistent with a 3∶1 ratio(χ^(2)_(3∶1)=0.14;P=0.71).In the assessment of 685 F_(2∶3)families,the segregation ratio among resistant individuals,resistant heterozygous individuals,and susceptible individuals conformed to a 1∶2∶1 ratio(χ^(2)_(1∶2∶1)=2.01;P=0.37),suggesting that Lr12 is a dominant gene and the population segregation follows Mendelian single-gene inheritance patterns.Genetic linkage map analysis localized the adult plant leaf rust resistance gene Lr12 between SSR molecular markers YK12817 and YK12928,within a genetic interval of 0.38 cM.This corresponds to a physical interval of 2.09 Mb within the physical range of 579.44 Mb to 581.53 Mb on chromosome 4BL of the Chinese Spring reference genome(IWGSC.Ref.V1.0).These findings provide a solid basis for predicting candidate genes.
作者
刘志杰
王新海
高璞
董瑞
李帅杰
张培培
刘大群
李在峰
LIU Zhijie;WANG Xinhai;GAO Pu;DONG Rui;LI Shuaijie;ZHANG Peipei;LIU Daqun;LI Zaifeng(Biological Control Center of Plant Diseases and Pests of Hebei Province,College of Plant Protection,Hebei Agricultural University,Baoding 071001,China)
出处
《华北农学报》
CSCD
北大核心
2024年第5期179-185,共7页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(32161143007)。
