摘要
分析了荷叶提取物(LLE)在抑制口腔细菌和改善牙龈炎症的有效性。使用不同质量浓度的LLE处理10种常见口腔细菌24 h,分别检测最小抑菌浓度(MIC)和抑菌圈直径。使用CCK-8法检测LLE对人牙龈上皮细胞活力的影响。将人牙龈上皮细胞分为对照组(Control)、脂多糖(LPS)组和LPS+1~500μg/mL LLE组,分别用1μg/mL LPS和不同质量浓度的LLE处理人牙龈上皮细胞24 h。RT-qPCR检测白介素(IL)-1β、IL-6、IL-8、肿瘤坏死因子-α(TNF-α)、细胞间黏附分子-1(ICAM-1)mRNA水平。Western blotting检测核因子-κB(NF-κB)p65和p38丝裂原活化蛋白激酶(MAPK)的磷酸化水平。结果显示,LLE对10种常见口腔细菌的MIC在300~500μg/mL之间。与Control组相比,LPS组人牙龈上皮细胞中IL-1β、IL-6、IL-8、TNF-α和ICAM-1 mRNA相对水平均升高,NF-κB p65和p38 MAPK磷酸化水平均升高(P<0.05)。与LPS组相比,LPS+50~500μg/mL LLE组人牙龈上皮细胞中IL-1β、IL-6、IL-8、TNF-α和ICAM-1 mRNA相对水平均呈LLE剂量依赖性降低,NF-κB p65和p38 MAPK磷酸化水平均呈LLE剂量依赖性降低(P<0.05)。本研究表明,LLE对10种常见口腔细菌和LPS诱导的人牙龈上皮细胞过度炎症具有抑制活性,其可能是口腔护理产品的有效添加成分。
This study aimed to analyze the effectiveness of Lotus leaf extract(LLE)in inhibiting oral bacteria and improving gingival inflammation.LLE of different mass concentrations was used to treat 10 common oral bacteria for 24 hours,and the minimum inhibitory concentration(MIC)and inhibition zone diameter were measured respectively.The CCK-8 method was used to detect the effect of LLE on the viability of human gingival epithelial cells.Human gingival epithelial cells were divided into control group(Control),lipopolysaccharides(LPS)group and LPS+1-500μg/mL LLE group.Human gingival epithelial cells were treated with 1μg/mL LPS and different mass concentrations of LLE respectively for 24 h.RT-qPCR were used to detect the mRNA levels of interleukin(IL)-1β,IL-6,IL-8,tumor necrosis factor-α(TNF-α),and intercellular adhesion molecule-1(ICAM-1).Western blotting were used to detect the phosphorylation levels of nuclear factor-κB(NF-κB)p65 and p38 mitogen-activated protein kinase(MAPK).The results show that the MICs of LLE against 10 common oral bacteria are 300-500μg/mL,respectively.Compared with the Control group,the relative mRNA levels of IL-1β,IL-6,IL-8,TNF-αand ICAM-1 in human gingival epithelial cells in the LPS group are all increased,and the phosphorylation levels of NF-κB p65 and p38 MAPK are all increased(P<0.05).Compared with the LPS group,the relative mRNA levels of IL-1β,IL-6,IL-8,TNF-αand ICAM-1 in human gingival epithelial cells in the LPS+50-500μg/mL LLE group are all decreased in a dose-dependent manner,and the phosphorylation levels of NF-κB p65 and p38 MAPK are decreased in a dose-dependent manner(P<0.05).This study shows that LLE has inhibitory activity against 10 common oral bacteria and LPS-induced excessive inflammation of human gingival epithelial cells,and it may be an effective ingredient in oral care products.
作者
刘娟丽
徐慧萍
刘艳芳
董振花
王美艳
Juanli Liu;Huiping Xu;Yanfang Liu;Zhenhua Dong;Meiyan Wang(Nursing Department of Puyang Medical College,Puyang,Henan 457000,China;The First Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450000,China;The Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450000,China;Department of Stomatology at Chengde Nursing Vocational College,Chengde,Hebei 067000,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2024年第10期1235-1242,共8页
China Surfactant Detergent & Cosmetics
关键词
荷叶提取物
口腔细菌
牙龈炎症
人牙龈上皮细胞
核因子-ΚB
Lotus leaf extract
oral bacteria
gingival inflammation
human gingival epithelial cells
nuclear factor-κB
