摘要
研究不同性型蓖麻(Ricinus communis L.)花粉的育性,尤其是雌性系的花粉育性,对于蓖麻杂交育种工作意义重大。本研究首先利用TTC染色法和花粉萌发法分别检测了镶嵌型雌性系、两性系共6种蓖麻种质材料花粉的生活力,随后通过扫描电子显微镜观察了他们的花粉形态,并利用SPSS软件分析了其形态特征值。结果显示:TTC染色和花粉萌发结果基本一致,不同材料间花粉生活力差异显著,蓖麻镶嵌型雌性系HCH3花粉生活力最大(96.97%)、镶嵌型雌性系HCH1花粉生活力次之。两种性型的花粉均呈现为单粒,长球形,赤道面观为长球形,极面观为三裂近圆形,中等大小(花粉极轴长为30.17~42.07μm),属于N_(3)P_(4)C_(3)型花粉。花粉外壁为网状纹饰,脊顶部具有密集的瘤状突起。然而,不同材料间花粉的极轴长、网眼直径、网脊宽度、单位面积网眼数形态值上差异显著。主成分析和聚类分析将6份蓖麻材料按花粉大小分为两类。本研究为蓖麻的花粉可育性和杂交选育提供了新的数据支撑。
It is of great significance for cross breeding to study the pollen fertility of castor(Ricinus communis L.)pollen of different sex types,especially pistillate lines.In this study,first of all,the pollen viability of six castor germplasm materials including the pistillate lines with a few interspersed staminate flowers(ISF)and monoecious lines was detected by TTC staining and pollen germination method,respectively.Then,the pollen morphology of these materials was observed by scanning electron microscopy,and the morphological characteristics were analyzed by SPSS software.The results showed that there were significant differences in the viability of pollen from different materials.The pollen viability of the ISF pistillate line HCH3 was the highest(96.97%),followed by the ISF pistil-late line HCH1.The pollens of two different sex types were single,long spherical shape,long spherical in the equa-torial plane view,three-split and nearly round in the polar plane view,medium in size(polar axis length 30.17-42.07μm),belonging to N_(3)P_(4)C_(3) type.The outer wall of pollen is reticular ornamented,and the murus has a dense tu-berculate form.However,there were significant differences in the polar axis length,mesh diameter,reticular ridge width and the numbers of mesh per unit area of pollen among different materials.Six castor materials were divided into two groups according to pollen size by principal component analysis and cluster analysis.This study provides new data support for pollen fertility and cross breeding of castor.
作者
杨俊芳
王宏伟
王宙
赵宜婷
王亚
张宏斌
张晋龙
黄晋玲
曹越
YANG Jun-fang;WANG Hong-wei;WANG Zhou;ZHAO Yi-ting;WANG Ya;ZHANG Hong-bin;ZHANG Jin-long;HUANG Jin-ling;CAO Yue(College of Agriculture,Shanxi Agricultural University,Taiyuan 030031,China;Institute of Industrial Crops,Shanxi Agricultural University,Taiyuan 030031,China;Finance Department of Shanxi Agricultural University,Taiyuan 030031,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2024年第5期1040-1047,共8页
Chinese Journal of Oil Crop Sciences
基金
山西省科技厅青年科学研究项目(20210302124364)
吕梁市重点研发项目(2022NYGG18,2021NYGG-2-89)
耐盐碱能源作物品种展示与示范(TYGC-32)。
关键词
雌性系
花粉
生活力
扫描电子显微镜
形态特征
pistillate line
pollen
vitality
scanning electron microscope
morphological characteristic
