摘要
Self-assembly of bacteria in electric fields is a promising route to fabricate biomaterials with reversible and specific structures.However,due to relatively less studies,our understanding of the self-assembly of bacteria in electric fields is still incomplete.Particularly,how different bacterial species behave differently in their fieldmediated self-assembly behavior remains to be disclosed.In this study,we choose four bacterial species,including Shewanella oneidensis,Pseudomonas aeruginosa,Bacillus subtilis and Lactococcus lactis as model systems,and investigate their self-assembly behavior in alternating-current(AC)electric fields for both diluted and concentrated suspensions.The phase diagrams in the plane of applied field strength vs frequency are obtained.The results show that in diluted suspensions,a transition sequence of isotropic–paranematic–string–columnar phases is observed in all strains as the field strength increases.Details of the assembled structures are quantitatively differentiated among different strains.In concentrated suspensions,besides the isotropic and paranematic phases,a higher ordered phase with interdigitating rectangular crystal domains(OIR)and an ordered phase with smectic A liquid crystal domains are observed for S.oneidensis and P.aeruginosa,respectively.Our findings shed new light on fabricating potential biomaterials by assembling cells of appropriately chosen bacterial species that have desired surface properties under AC electric fields.
作者
Yan-Ran Li
Yi-Wu Zong
Hong Zhang
Jing-Chao Zhang
Chun-Ying Feng
Jian-Jun Qiao
Hao Song
Kun Zhao
李艳冉;宗奕吾;张红;张静超;冯春迎;乔建军;宋浩;赵坤(Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;Petrochemical Research Institute of Petrochina Co.,Ltd.,Beijing 102206,China;Center for Medical Genetics,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu 611731,China;Jiangsu Key Laboratory of Sericultural and Animal Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang 212100,China;Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,Sericultural Scientific Research Center,Chinese Academy of Agricultural Sciences,Zhenjiang 212100,China;Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China;The Sichuan Provincial Key Laboratory for Human Disease Gene Study and The Institute of Laboratory Medicine,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu 610054,China;Institute of Fundamental and Frontier Sciences,University of Electronic Science and Technology of China,Chengdu 610054,China)
基金
supported by the National Key R&D Program of China(Grant No.2023YFC3402401)
the National Natural Science Foundation of China(Grant No.12374206)。
