摘要
Background::Rheumatoid arthritis(RA)is a chronic autoimmune inflammatory disease.Its pathogenesis is not fully understood,and early diagnosis is challenging owing to the lack of effective biomarkers.This study aimed to analyze different samples to identify potential biomarkers and therapeutic targets.Methods::Microarray datasets of RA,osteoarthritis(OA),and healthy control(HC)were downloaded from the Gene Expression Omnibus database.R software was used to identify differentially expressed genes(DEGs),which were visualized using volcano and heat maps.Venn diagrams,principal component analysis,gene set enrichment analysis,gene ontology,and Kyoto Encyclopedia of genes and genomes were used to analyze the data.A protein–protein interaction network was constructed,and synovial tissues from patients with RA and OA were collected for verification using the collagen-induced arthritis mouse model.Results::More DEGs were found in synovial tissues than in peripheral blood mononuclear cells or fibroblast-like synoviocytes.Principal component analysis revealed significant differences between the RA and OA samples,highlighting the unique advantages of synovial tissue.Enrichment analysis revealed that metabolic and cytokine signaling pathways play crucial roles in the development of RA.Further analysis of the four synovial datasets identified 54 DEGs,of which signaling lymphocytic activation molecule family(SLAMF)8 was identified as the key molecule.SLAMF8 levels were increased in the synovial tissue of patients with RA compared to those of patients with OA(0.38±0.19 vs.12.40±1.66),and SLAMF8 levels were similarly elevated in collagen-induced arthritis model mice compared with those in the healthy mice(1.13±0.47 vs.9.05±2.52).Conclusions::This study established the unique advantages of synovial tissue for RA research and identified metabolic and cytokine signaling pathways as important for RA development.Thus,SLAMF8 may be a potential therapeutic target for RA.
基金
National Natural Science Foundation of China,Grant/Award Numbers:82071834,82101896,82271839
Dalian Medical University Interdisciplinary Research Cooperation Project Team Funding,Grant/Award Number:JCHZ2023010。
