摘要
为了明确油棕LOX基因种类、特性及其在油棕生长发育中的调控作用,本研究以拟南芥LOX蛋白序列为标签,在油棕全基因组数据库中BLAST同源LOX蛋白序列,并通过CDD和PFAM数据库对LOX蛋白序列进行分析,利用生物信息学对油棕LOX家族成员进行染色体定位、进化树、基因结构及保守结构域等可视化分析。结果表明,共获得75个油棕LOX基因,其中有61个基因在油棕的15条染色体上分布不均匀,在12号染色体上分布的基因最多,为10个;基因长度范围为1550~99179 bp,外显子数目为1~24个;油棕LOX基因家族共含有10个motif保守基序;通过预测蛋白的二级结构,发现EgLOX蛋白均存在α-螺旋、β-转角、无规卷曲及延伸链这4种结构,其中57个蛋白以无规卷曲为主要结构,18个蛋白以α-螺旋为主要结构,而β-转角及延伸链所占比例均较少;通过表达模式分析发现EgLOX基因在油棕中果皮、果仁、根、叶、茎和花中均有不同程度的表达,具有组织和时空表达特异性。基因重复和组织表达特异性是油棕EgLOX基因家族的两大特点,推测该家族基因在油棕脂质降解过程中起调控作用。本研究为油棕LOX的功能研究提供一定的参考。
In order to clarify the types,characteristics,and regulatory roles of oil palm LOX genes in the growth and development of oil palm,this study used Arabidopsis LOX protein sequences as labels and BLAST homologous LOX protein sequences in the oil palm genome database.The LOX protein sequences were analyzed using CDD and PFAM databases.Bioinformatics was used to visualize chromosomal localization,evolutionary tree,gene structure,and conserved domains of oil palm LOX family members.The results showed that 75 LOX genes of oil palm were obtained,of which 61 genes were unevenly distributed on 15 chromosomes of oil palm,and 10 genes were most distributed on chromosome 12.The length of the gene ranged from 1550 bp to 99179 bp,and the number of exon ranged from 1 to 24.The LOX gene family of oil palm contains 10 motif conserved motifs.By predicting the secondary structures of the proteins,it was found that all EgLOX proteins have four types of structures:α-helix,β-turn,random coil,and extended strand,of which 57 proteins have random coil as the main structure,18 proteins haveα-helix as the main structure,and the proportion ofβ-turn and extended strand is small.Through expression pattern analysis,it was found that the EgLOX gene is expressed to varying degrees in the skin,kernels,roots,leaves,stems,and flowers of oil palm,with tissue and spatiotemporal expression specificity.Antifreeze protein and tissue expression specificity are two major characteristics of the oil palm EgLOX gene family,which may play a regulatory role in the process of oil palm lipid degradation.This study provides a certain reference for the functional research of oil palm LOX.
作者
周丽霞
吴秋妃
曾春茹
曹红星
李睿
Zhou Lixia;Wu Qiufei;Zeng Chunru;Cao Hongxing;Li Rui(Hainan Provincial Key Laboratory of Tropical Oil Crops Biology,Coconut Research Institute,Chinese Academy of Tropical Agricultural Science,Wenchang,571339)
出处
《分子植物育种》
CAS
北大核心
2024年第20期6719-6730,共12页
Molecular Plant Breeding
基金
海南省重点研发计划项目(No.ZDYF2022XDNY207)
中国热带农业科学院基本科研业务费专项(No.1630-152022001)共同资助。
