摘要
目的通过检测慢性鼻窦炎(CRS)引起的嗅觉障碍患者嗅区黏膜上皮组织中环磷腺苷效应元件结合蛋白2(CREB2)与嗅觉标记蛋白(OMP)的表达情况,探讨其与CRS患者嗅觉障碍的相关性。方法纳入2021年9月—2022年3月徐州医科大学附属医院收治的25例CRS伴嗅觉障碍的患者作为观察组,另选15例鼻中隔偏曲嗅觉正常患者作为对照。术前采用嗅棒TDI评分检测2组患者的嗅觉功能,术中取嗅区黏膜作为组织标本,通过免疫组化染色和Western blot检测嗅区黏膜上皮组织中CREB2与OMP表达水平,并分析其与嗅觉障碍的相关性。结果观察组TDI分值显著低于对照组(P<0.05)。免疫组化和Western blot结果表明:观察组嗅区黏膜上皮组织中CREB2与OMP阳性细胞数和蛋白表达量与对照组相比显著下降(P<0.01)。嗅区黏膜上皮组织中CREB2、OMP表达水平与TDI分值呈正相关(r=0.974,P<0.01;r=0.959,P<0.01)。结论CREB2和OMP在CRS伴嗅觉障碍患者嗅区黏膜上皮组织中表达降低,表达水平与TDI分值呈正相关。CRS患者嗅区黏膜上皮组织中CREB2表达减少可能与嗅觉障碍的分子机制有关。
Objective To explore the correlation between the expression of cAMP response element-binding protein 2(CREB2)and olfactory marker protein(OMP)in the olfactory mucosal epithelium and olfactory disorders in patients with chronic rhinosinusitis(CRS).Methods A total of 25 CRS patients with olfactory disorders who were admitted to the Affiliated Hospital of Xuzhou Medical University from September 2021 to March 2022 were selected as an observation group.Meanwhile,additional 15 patients with nasal septum deviation and normal olfaction were chosen as a control group.Preoperative olfactory function was assessed in both groups using the sniffin′sticks TDI scoring system.During surgery,olfactory mucosal tissues were collected as specimens.The levels of CREB2 and OMP in the olfactory mucosal epithelium were detected using immunohistochemistry and Western blot,and their correlation with olfactory function was analyzed.Results The TDI scores in the observation group were significantly lower than those in the control group(P<0.05).Immunohistochemistry and Western blot results showed that the number of CREB2 and OMP positive cells and their protein expression levels in the olfactory mucosa significantly decreased in the observation group,compared with the control group(P<0.01).The levels of CREB2 and OMP in the olfactory mucosal epithelium were positively correlated with TDI scores(r=0.974,P<0.01;r=0.959,P<0.01).Conclusions The decreased expression of CREB2 and OMP in the olfactory mucosal epithelium of CRS patients with olfactory disorders is positively correlated with TDI scores.Therefore,reduced expression of CREB2 in the olfactory mucosal epithelium may be associated with the molecular mechanism of olfactory disorders in CRS patients.
作者
王浩
邵宇迪
肖朝球
张燕灵
李巍
WANG Hao;SHAO Yudi;XIAO Chaoqiu;ZHANG Yanling;LI Wei(Department of Otolaryngology,Head and Neck Surgery,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221002,China;The First School of Clinical Medicine,Xuzhou Medical University,Xuzhou,Jiangsu 221004)
出处
《徐州医科大学学报》
CAS
2024年第8期613-617,共5页
Journal of Xuzhou Medical University
关键词
慢性鼻窦炎
嗅觉障碍
环磷腺苷效应元件结合蛋白
嗅觉标记蛋白
嗅区黏膜上皮
chronic rhinosinusitis
olfactory disorders
cAMP response element-binding protein 2
olfactory marker protein
olfactory mucosal epithelium
