摘要
目的观察循经选穴针刺对对氯苯丙氨酸(PCPA)致失眠大鼠前额叶相关蛋白激酶表达的影响,探讨针刺改善失眠的作用机制。方法将60只大鼠随机分为空白组、模型组、假针刺组、针刺组和西药组,每组12只。采用腹腔注射PCPA复制失眠大鼠模型,针刺组针刺“百会”“神门”(双侧)、“三阴交”(双侧),假针刺组仅将针头固定于相应穴位但不刺入皮肤,模型组仅固定,每日30 min,西药组予艾司唑仑溶液灌胃,连续7 d。空白组不进行处理。戊巴比妥钠翻正实验测定大鼠睡眠潜伏期和睡眠时间,ELISA检测血浆褪黑素(MT)含量,RT-PCR检测前额叶组织钙调蛋白依赖性蛋白激酶(CaMK)Ⅱ、蛋白激酶C(PKC)、p38丝裂原活化蛋白激酶(MAPK)mRNA表达,Western blot检测前额叶组织CaMKⅡ、PKC、p38MAPK蛋白表达。结果与空白组比较,模型组大鼠睡眠潜伏期显著延长、睡眠时间显著缩短(P<0.01),血浆MT含量显著降低(P<0.01),前额叶组织CaMKⅡ、PKC mRNA及蛋白表达显著降低(P<0.01),p38MAPK mRNA及蛋白表达显著升高(P<0.01);与模型组比较,针刺组和西药组大鼠睡眠潜伏期显著缩短、睡眠时间显著延长(P<0.01),血浆MT含量显著升高(P<0.01),前额叶组织CaMKⅡ、PKC mRNA及蛋白表达显著升高(P<0.05),p38MAPK mRNA及蛋白表达显著降低(P<0.01);与假针刺组比较,针刺组和西药组睡眠潜伏期显著缩短、睡眠时间显著延长(P<0.01),血浆MT含量显著升高(P<0.01),前额叶组织PKC mRNA表达显著升高(P<0.01),p38MAPK mRNA表达显著降低(P<0.01)。针刺组与西药组各指标差异无统计学意义(P>0.05)。结论循经选穴针刺可能通过调节失眠大鼠前额叶相关蛋白激酶表达,缩短大鼠睡眠潜伏期、延长睡眠时间,从而改善睡眠状态。
Objective To observe the effects of acupuncture at acupoints along the meridians on the expression of frontal lobe associated protein kinase in pchlorophenylalanine(PCPA)induced insomnia rats;To explore the mechanism of acupuncture in improving insomnia.Methods Totally 60 rats were randomly divided into blank group,model group,sham acupuncture group,acupuncture group and Western medicine group,with 12 rats in each group.An insomnia model rat was induced by intraperitoneal injection of PCPA.The acupuncture group received acupuncture at“Baihui”,“Shenmen”(bilateral),and“Sanyinjiao”(bilateral),while the sham acupuncture group only fixed the needle at the corresponding acupoints but did not penetrate the skin,while the model group was only fixed for 30 minutes,the Western medicine group was given a solution of estazolam by gavage for 7 consecutive days,the blank group was not treated.The pentobarbital sodium reversal experiment was used to detect the sleep latency and sleep time of rats,ELISA was used to detect the content of melatonin(MT)in plasma,RT-PCR was used to detect the mRNA expressions of calmodulin dependent protein kinase(CaMK)Ⅱ,protein kinase C(PKC)and p38 mitogen activated protein kinase(MAPK)in frontal lobe tissue,and Western blot was used to detect the protein expressions of CaMKⅡ,PKC and p38MAPK in frontal lobe tissue.Results Compared with the blank group,the sleep latency of the model group rats were significantly prolonged and the sleep time were significantly shortened(P<0.01),the content of MT in plasma was significantly reduced(P<0.01),and the mRNA and protein expressions of CaMKⅡand PKC in frontal lobe tissue were significantly decreased(P<0.01),the mRNA and protein expressions of p38MAPK were significantly increased(P<0.01).Compared with the model group,the sleep latency were significantly shortened and the sleep time were significantly prolonged in the acupuncture group and the Western medicine group(P<0.01),the plasma MT content significantly increased(P<0.01),the mRNA and pr
作者
吴雪芬
朱洲
莫倩
张小珊
施杨婉琳
吴艳茹
贾晓琪
杨孝芳
WU Xuefen;ZHU Zhou;MO Qian;ZHANG Xiaoshan;SHI Yangwanlin;WU Yanru;JIA Xiaoqi;YANG Xiaofang(School of Acupuncture and Massage,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2024年第10期115-120,共6页
Chinese Journal of Information on Traditional Chinese Medicine
基金
贵州省科技计划项目-自然科学(黔科合基础-ZK[2023]一般423)
贵州省中医药管理局中医药、民族医药科学技术研究专项课题(QZYY-2024-033)
贵州中医药大学博士启动基金([2022]13号)。
关键词
失眠
循经选穴
针刺
蛋白激酶
大鼠
insomnia
acupuncture at acupoints along the meridians
acupuncture
protein kinase
rats
