摘要
透明质酸酶(hyaluronidase, HAase)在自然界分布广泛,从复杂的高等动物到低等的细菌中均有发现,它能使透明质酸(hyaluronic acid, HA)分解,从而造成保湿成分的流失。利用从天然生长礁膜(Monostroma nitidum)中提取的硫酸鼠李糖(rhamnan sulfate, RS)研究其对HAase活性抑制的机理有利于今后海藻多糖在保湿护肤及健康产品方面的开发利用。红外吸收光谱分析RS、HAase及两者混合物,结果表明生成的复合物中存在与RS相同的硫酸基;RS-HAase复合物中沉淀的形成,与硫酸基和pH值有关;双缩脲反应测定蛋白质,显示硫酸基参与了HAase和RS复合物的生成,RS抑制HAase活性强弱取决于RS中硫酸基含量的多少;酶抑制动力学分析表明,RS对HAase的抑制作用属于竞争性抑制。
Hyaluronidase(HAase)is widespread throughout the natural world,ranging from complex higher animals to simpler bacterial species.It catalyzes the degradation of hyaluronic acid,resulting in the loss of moisturizing components.The research into the inhibitory mechanism of rhamnan sulfate(RS),extracted from naturally grown Monostroma nitidum,on HAase activity is beneficial for the future development and utilization of algal polysaccharides in moisturizing skincare and health products.The IR analysis of RS,HAase and their mixture revealed the presence of sulfate groups identical to those in RS within the formed complexes;the formation of precipitation in the RS-HAase complex was found to be associated with sulfate groups and pH values;the biuret protein assay indicated that sulfate groups were involved in the formation of HAase and RS complexes,and the degree of inhibition of HAase activity by RS was dependent on the sulfate group content of RS;The analysis of enzyme inhibition kinetics demonstrated that the inhibition of HAase by the RS was of a competitive inhibition nature.
作者
张洋
ZHANG Yang(School of Food and Biological Engineering,Wuhu Institute of Technology,Wuhu 241003,China)
出处
《长春师范大学学报》
2024年第6期90-94,共5页
Journal of Changchun Normal University
基金
安徽省高校自然科学研究重点项目“海洋绿藻中提取成分的相关活性研究”(KJ2020A0921)。
关键词
硫酸鼠李糖
透明质酸酶
硫酸基含量
抑制作用
礁膜
rhamnan sulfate
hyaluronidase
sulfate group content
inhibition
Monostroma nitidum
