摘要
目的 探究乙型肝炎病毒X基因(HBx)对HepG2细胞中Caspase-1介导的肝细胞增殖的影响。方法 用脂质体转染法将HBx真核表达载体pCDNA3.1-HBx瞬时转入HepG2细胞,以阴性载体转染的HepG2细胞(NC)为对照。转染后72 h收集细胞,通过RT-PCR检测HBx的表达,通过qPCR和Western blot法检测Caspase-1的mRNA和蛋白质表达,并通过CCK-8检测HepG2-HBx和HepG2-NC的增殖情况。结果 RT-PCR证实HBx成功转染HepG2细胞株,HepG2-HBx细胞中Caspase-1的mRNA和蛋白表达水平显著低于HepG2-NC组。HepG2-HBx细胞的增殖能力显著高于HepG2-NC组。结论 HBx通过抑制Caspase-1的表达诱导HepG2细胞增殖。
Objective To investigate the effect of hepatitis B virus X gene(HBx)on the proliferation of HepG2 cells mediated by Caspase-1.Methods The pcDNA 3.1 vector loading Hbx(pcDNA3.1-HBx)was transfected into HepG2 cells using lipofectamine,with those transfected with negative vector as negative control(NC).Cells were collected 72h after transfection.The expression of HBx was detected by reverse transcription-polymerase chain reaction(RT-PCR).The mRNA and protein levels of Caspase-1 were detected by quantitative polymerase chain reaction(qPCR)and Western blot,respectively.The proliferation of HepG2 cells transfected with pcDNA3.1-HBx and NC was assessed by CCK-8 assay.Results Transfection of pcDNA3.1-HBx was success with the verification by RT-PCR.The mRNA and protein levels of Caspase-1 were significantly lower in HepG2 cells transfected with pcDNA3.1-HBx than those transfected with NC.The proliferative rate was significantly higher in HepG2 cells transfected with pcDNA3.1-HBx than those transfected with NC.Conclusion HBx promotes the proliferation of HepG2 cells by downregulating Caspase-1.
作者
韦武均
王春芳
钟丽梅
黄晶晶
唐霏林
黄艳
黄英新
陆艳珍
WEI Wujun;WANG Chunfang;ZHONG Limei(Department of Laboratory Medicine,Affiliated Hospital of Youjiang Medical University for Nationalities,Guangxi,Baise 533000,China;不详)
出处
《河北医药》
CAS
2024年第19期2927-2930,共4页
Hebei Medical Journal
基金
国家自然科学基金项目(编号:81960303)
广西卫生健康委员会自筹经费科研课题(编号:Z20211114,Z20190202)
百色市科学研究与技术开发计划项目(编号:百科20213242)
广西高校中青年教师科研基础能力提升项目(编号:2021KY0538)
中医药自筹经费科研课题(编号:GXZYL20220304)
广西肝胆疾病分子病理学重点实验室开放课题(编号:GXZDSYS-009)。
