摘要
目的探讨人参皂苷Rc(Ginsenoside Rc,简称Rc)对酒精性肝病的保护作用及其作用机制。方法采用50 mmol/L乙醇(EtOH)刺激小鼠肝细胞(AML-12)12 h后,采用不同浓度Rc处理24 h。Western blot方法检测AML-12细胞中P2X7r、NLRP3和IL-23蛋白表达情况;RT-qPCR测定AML-12细胞中SREBP1、Lipin-1、P2X7r、NLRP3的mRNA表达情况;通过ELISA法分析肝细胞上清液中IL-1β和IL-18水平。结果与对照组比较,EtOH能够诱导AML-12细胞脂质变性和炎症因子释放,Rc能够有效下调P2X7r、NLRP3和IL-23的蛋白或mRNA表达,抑制EtOH诱导AML-12细胞中SREBP1和Lipin-1的mRNA表达,抑制EtOH诱导AML-12细胞中IL-1β和IL-18向胞外释放(均P<0.001)。结论Rc能够抑制酒精性肝病进程,作用机制可能与抑制P2X7r-NLRP3信号通路有关。
Objective To investigate the protective effect of Ginsenoside Rc(Rc)on alcoholic liver disease.Method Mice hepatocytes line(AML-12)were stimulated with 50 mmol/L ethanol(EtOH)for 12 hours,and then treated with different concentrations of Rc for 24 hours.Western blot was used to detect the protein expressions of P2X7r,NLRP3 and IL-23 in AML-12 cells.The mRNA expressions of SREBP1,Lipin-1,P2X7r and NLRP3 in AML-12 cells were determined by RT-qPCR.The levels of IL-1βand IL-18 in hepatocyte supernatants were analyzed by ELISA.Results EtOH induced the release of lipid degeneration and inflammatory factors in AML-12 cells,and Rc could effectively downregulate the protein or mRNA expression of P2X7r,NLRP3 and IL-23.Meanwhile,Rc could inhibit the mRNA expressions of SREBP1 and Lipin-1 in EtOH-stimulated AML-12 cells.In addition,Rc could inhibit the extracellular release of IL-1βand IL-18 in EtOH-stimulated AML-12 cells.Conclusion Rc could inhibit the progression of alcoholic liver disease via inhibiting of P2X7r-NLR P3 signaling pathway.
作者
张金金
冯亓垣
孙海明
宋健
ZHANG Jinjin;FENG Qiyuan;SUN Haiming;SONG Jian(Pharmacy College of Beihua University,Jilin 132013,China)
出处
《北华大学学报（自然科学版）》
CAS
2024年第6期744-748,共5页
Journal of Beihua University（Natural Science）
基金
国家自然科学基金项目(82304848)
吉林省科技发展计划项目(20240305068YY)。
