摘要
目的:探讨红光LED照射对自然老化SKH-1小鼠皮肤的影响。方法:选取20只12月龄SKH-1小鼠,随机分为四组,使用红光LED仪对各组小鼠以0 J/cm^(2)、14.4 J/cm^(2)、32.4 J/cm^(2)和50.4 J/cm^(2)的能量密度连续照射60 d。在第0天、第10天、第30天和第60天,通过拍摄大体照片和皮肤镜照片记录皮肤外观变化,从皮肤粗糙、皮肤皱纹和皮肤紧致三个方面进行皮肤老化等级评分,检测角质层含水量(Water content of the stratum corneum,WCSC)、经皮失水率(Transepidermal water loss,TEWL)评估皮肤屏障功能,行光学相干断层扫描成像技术(Optical coherence tomography,OCT)检测真皮层信号强度,通过HE染色与Masson染色比较四组小鼠的皮肤组织结构变化。结果:与0 J/cm^(2)组相比,肉眼和皮肤镜下均观察到红光照射组小鼠的皮肤更光滑紧致,且皮肤老化等级评分表明在红光照射后小鼠皮肤粗糙、皮肤皱纹和皮肤紧致程度明显改善。红光照射不仅改善皮肤外观,而且能显著改善WCSC和TEWL。OCT结果表明红光照射增加了真皮层厚度和真皮信号强度。皮肤组织HE染色结果表明红光照射增加表皮厚度,维持表皮层细胞形态正常且排列紧密。Masson染色结果显示红光照射的小鼠真皮胶原含量显著增多,胶原纤维排列整齐紧密。红光LED照射组的以上指标的改善作用随能量密度的增大而增强,尤其是50.4 J/cm^(2)组的作用最为显著,且改善作用持续至照光第60天。结论:红光LED可显著改善自然老化SKH-1小鼠皮肤外观和皮肤屏障功能,增加表皮的厚度和真皮胶原含量,维持胶原纤维的紧密排列,为LED光源在皮肤老化的临床应用提供了实验依据。
Objective To investigate the effect of red light-emitting LED on the skin of intrinsically aged SKH-1 mice.Methods Twenty 12-month-old SKH-1 mice were selected and randomly divided into four groups,and each group was irradiated with light doses of 0 J/cm^(2),14.4 J/cm^(2),2232.4 J/cm and 50.4 J/cm for 60 consecutive days using a red light LED instrument.On days 0,10,30 and 60,changes in skin appearance were recorded by taking gross photographs and dermoscopic photographs,and a skin aging rating scale was scored in terms of skin roughness,skin lines and skin firmness.Skin barrier function was measured by the water content of the stratum corneum(WCSC)and the transepidermal water loss(TEWL).Optical coherence tomography(OCT)was performed to detect the signal intensity in the dermis,and the structural changes in the skin of the four groups of mice were compared by HE staining and Masson staining.Results 2 Compared to the 0 J/cm group,smoother and firmer skin was observed both visually and dermoscopically in the red light-treated mice,and and skin aging rating scale showed a significant improvement in skin roughness,skin wrinkles and skin firmness after red light irradiation.Red light irradiation not only improved skin appearance,but also significantly improved WCSC and TEWL.OCT results showed that red light irradiation increased the dermal thickness and dermal signal intensity.HE staining of skin tissues showed that red light irradiation increased epidermal thickness and maintained normal and tightly arranged cells in the epidermal layer.The Masson staining results showed that the dermal collagen content was significantly increased and collagen fibers were neatly arranged in the dermis of mice treated with red light.The improvement of the above indicators in the red LED irradiation group 2 was enhanced with the increase of energy density,especially the effect of 50.4 J/cm group was the most significant,and the improvement lasted until the 60th day of illumination.Conclusion Red LED can significantly improve the skin a
作者
康紫薇
陈琦
申抒展
王秀丽
王佩茹
KANG Ziwei;CHEN Qi;SHEN Shuzhan;WANG Xiuli;WANG Peiru(Institute of Photomedicine,Shanghai Skin Disease Hospital,Tongji University School of Medicine,Shanghai 200443,China)
出处
《中国美容医学》
CAS
2024年第10期1-6,共6页
Chinese Journal of Aesthetic Medicine
基金
上海市科学技术委员会“科技创新行动计划”自然科学基金(名称:皮肤淋巴管重塑在光动力抗哀老中的作用及机制研究
编号:22ZR1455400)。
