摘要
目的采用荧光淬灭光谱法研究金匮肾气丸及其拆方与牛血清白蛋白(BSA)相互作用的规律。方法根据304 K、307 K和313 K三个温度下金匮肾气丸及其拆方对BSA的荧光淬灭作用,利用Stern-Volmer方程和双对数方程对实验数据进行处理,分析金匮肾气丸及其拆方与BSA相互作用的荧光淬灭类型和结合常数。结果随着金匮肾气丸及其拆方中定量指标成分浓度增加,BSA的荧光强度逐渐减小;同一温度下,金匮肾气丸阴阳双补组与BSA相互作用的结合常数Ka值大于含相同定量指标成分的补阳组或补阴组,含“三泻”药物(茯苓、泽泻、牡丹皮)的组与BSA相互作用的结合常数K_(a)值大于不含“三泻”药物的组。结论金匮肾气丸及其拆方均能与BSA结合,使得BSA的荧光淬灭,其淬灭类型均以静态淬灭为主;金匮肾气丸阴阳双补组与BSA的相互作用强于含相同定量指标成分的补阳组或补阴组,“三泻”药物的加入使得这种相互作用进一步增强。
Objective To determine the interaction of Jinkui Shenqi pills and their disassembled formulas with bovine serum albumin(BSA)with fluorescence quenching spectroscopy.Methods According to the fluorescence quenching effect of Jinkui Shenqi pills and their disassembled formulas on BSA at 304 K,307 K,and 313 K temperature,Stern-Volmer equation and double log equation were used to analyze the fluorescence quenching type and binding constant of Jinkui Shenqi pills and their disassembled formulas with BSA.Results With the increase of the concentration of quantitative index components among Jinkui Shenqi pills and their disassembled foumulas,the fluorescence intensity of BSA decreased gradually.At the same temperature,the binding constant(the K_(a) value),of the interaction of the Yin-Yang double supplementing group of Jinkui Shenqi pills with BSA was higher than that of Yang-supplementing group or the Yin-supplementing group with the same quantitative index components;the Ka value of the interaction of the group containing purgatives(poria cocos,rhizoma alismatis,and cortex moutan)with BSA was higher than that of the group without purgatives.Conclusion Jinkui Shenqi pills and their disassembled formulas can combine BSA and quench the fluorescence of BSA,with static quenching as the main type.The interaction of Yin-Yang double supplementing group of Jinkui Shenqi pills with BSA is stronger than that of Yang-supplementing group or Yin-supplementing group with the same quantitative index components,and addition of“three purgatives”further enhances this interaction.
作者
张敏
孟晶莹
王亚楠
邬瑞光
ZHANG Min;MENG Jing-ying;WANG Ya-nan;WU Rui-guang*(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488)
出处
《中南药学》
CAS
2024年第9期2363-2368,共6页
Central South Pharmacy
基金
国家中医药管理局高水平建设学科项目(No.zyyzdxk-2023272)
北京中医药大学科研发展基金项目(No.2024-ZXFZJJ-005)。
关键词
金匮肾气丸
补阳组
补阴组
牛血清白蛋白
荧光淬灭光谱法
Jinkui Shenqi pill
Yang-supplementing group
Yin-supplementing group
bovine serum albumin
fluorescence quenching spectroscopy
