摘要
[目的]本研究旨在明确黑龙江省大豆田反枝苋(Amaranthus retroflexus L.)种群对氟磺胺草醚的抗药性水平,并研究其产生抗性及多抗性的分子机制。[方法]利用琼脂法快速测定采自黑龙江省嫩江县的3个反枝苋种群对氟磺胺草醚的抗性水平。通过盆栽法筛选出抗氟磺胺草醚/噻吩磺隆的反枝苋植株,并进行原卟啉原氧化酶(protoporphyrinogenⅨoxidase, PPO)和乙酰乳酸合酶(acetolactate synthase, ALS)的靶标基因测序。[结果]相比于敏感种群,黑龙江省3个种群对氟磺胺草醚的相对抗性倍数(resistance index, RI)分别为83.6、83.8和93.4;位于反枝苋PPO2高度保守区域编码128位氨基酸的核苷酸由AGG突变为GGG,导致精氨酸被替换为甘氨酸(Arg-128-Gly)。3个抗氟磺胺草醚的反枝苋种群均对ALS抑制剂类除草剂产生多抗性。对als基因测序后发现,与敏感种群相比,各抗性种群10个单株的ALS保守区域均发生多个不同位点(Ala-205-Val、Asp-376-Glu、Trp-574-Leu和Ser-653-Thr)及组合的氨基酸取代方式,各种群突变位点和杂合度多样。[结论]PPO2的Arg-128-Gly氨基酸取代可能是黑龙江3个反枝苋种群对氟磺胺草醚产生抗性的主要原因,3个种群同时对ALS抑制剂类除草剂存在靶标位点突变导致的多抗性。
[Objectives]The paper aimed to determine the resistance level of Amaranthus retroflexus L.populations to fomesafen collected from soybean fields in Heilongjiang Province,and to study the molecular mechanism of fomesafen resistance and multiple resistance.[Methods]Three populations from Nenjiang County in Heilongjiang Province were evaluated for resistance level to fomesafen by agar-based rapid bioassay.The plants that survived fomesafen or thifensulfuron-methyl treatment in pots at the field recommend dose were supposed to be the resistant ones,and their full-length sequences of protoporphyrinogenⅨoxidase genes and acetolactate synthase gene were sequenced.[Results]Compared with the sensitive population,the relative resistance index(RI)of the three populations to fomesafen were 83.6,83.8 and 93.4,respectively.It was found that the nucleotides encoding amino acids at 128 in the highly conserved PPO2 region were mutated from AGG to GGG,resulting in the substitution of arginine to glycine(Arg^(-1)28-Gly).Additionally,all three populations exhibited multiple resistance to ALS inhibitors.The als gene sequencing showed that single or combined amino acid substitutions at multiple sites(Ala-205-Val,Asp-376-Glu,Trp-574-Leu,and Ser-653-Thr)occurred in the ALS conserved region of the three resistant populations,with a diverse heterozygosity.[Conclusions]Arg^(-1)28-Gly amino acid substitution of PPO2 might be the main resistance mechanism of A.retroflexus L.populations to fomesafen.All the three A.retroflexus L.populations showed multiple resistance to ALS inhibitors,and the resistance mechanism was mainly due to target site ALS mutations.
作者
吴群
韦建国
郭嘉颖
张战战
冯致科
陈金奕
WU Qun;WEI Jianguo;GUO Jiaying;ZHANG Zhanzhan;FENG Zhike;CHEN Jinyi(Laboratory of Herbicide Toxicology and Resistance,Nanjing Agricultural University,Nanjing 210095,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2024年第5期873-879,共7页
Journal of Nanjing Agricultural University
基金
国家重点研发计划项目(2023YFD1400503)。
