摘要
目的探究槲皮素(Quer)诱导乳腺癌细胞凋亡的机制。方法将人乳腺癌细胞MCF-7分为4组,分别为对照组、实验组、阴性对照(NC)组和miR-373-3pmimics组。对照组细胞在正常条件下培养;实验组用80μmol·L^(-1)槲皮素处理;NC组用80μmol·L^(-1)槲皮素处理后,转染50nmol·L^(-1)miR-373-3p的阴性对照;miR-373-3pmimics组用80μmol·L^(-1)槲皮素处理,转染50nmol·L^(-1)miR-373-3p。用实时定量聚合酶链反应(qRT-PCR)检测各组细胞中微小核糖核酸-373-3p(miR-373-3p)、硫氧还蛋白互作蛋白(TXNIP)、NOD样受体热蛋白结构域蛋白3(NLRP3)、B细胞淋巴瘤-2(Bcl-2)和Bcl-2相关X蛋白(Bax)的表达情况,用原位末端标记法(Tunel)实验检测各处理组细胞的凋亡率。结果对照组、实验组、NC组、miR-373-3pmimics组的miR-373-3p相对表达水平分别为1.00±0.15、0.61±0.08、0.67±0.05和1.19±0.16,TXNIPmRNA相对表达水平分别为1.00±0.12、1.83±0.21、1.71±0.19和1.26±0.14,NLRP3mRNA相对表达水平分别为1.00±0.11、0.73±0.06、0.65±0.08和1.27±0.15,Bcl-2mRNA相对表达水平分别为1.00±0.15、0.43±0.06、0.37±0.04和0.83±0.11,BaxmRNA相对表达水平分别为1.00±0.12、2.13±0.25、1.96±0.21和1.38±0.17,凋亡率分别为(100.00±13.55)%、(217.24±25.13)%、(196.37±21.53)%和(134.71±16.42)%。实验组的上述指标与对照组比较,miR-373-3pmimics组的上述指标与NC比较,在统计学上差异均有统计学意义(P<0.05,P<0.01,P<0.001)。结论槲皮素通过调控miR-373-3p/TXNIP/NLRP3通路诱导乳腺癌细胞凋亡。
Objective To explore the mechanism of quercetin(Quer)inducing apoptosis of breast cancer cells.Methods Human breast cancer cells MCF-7 were divided into 4 groups:control group,experimental group,negative control(NC)group and miR-373-3p mimics group.The control group was cultured under normal conditions;the experimental group was treated with 80μmol·L^(-1)quercetin;NC group was treated with 80μmol·L^(-1)quercetin and transfected with 50 nmol·L^(-1)miR-373-3p negative control;miR-373-3p mimics group was treated with 80μmol·L^(-1)quercetin and transfected with 50 nmol·L^(-1)miR-373-3p.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect microRNA 373-3p(miR-373-3p),thioredoxin-interacting protein(TXNIP),NOD-like receptor family pyrin domain containing protein 3(NLRP3),B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax).Terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling(Tunel)assay was used to detect the apoptosis rate of each treatment group.Results The relative expression levels of miR-373-3p in control group,experimental group,NC group and miR-373-3p mimics group were 1.00±0.15,0.61±0.08,0.67±0.05 and1.19±0.16,respectively;the relative expression levels of TXNIP mRNA were 1.00±0.12,1.83±0.21,1.71±0.19 and 1.26±0.14,respectively;the relative expression levels of NLRP3 mRNA were 1.00±0.11,0.73±0.06,0.65±0.08 and 1.27±0.15,respectively;the relative expression levels of Bcl-2 mRNA were1.00±0.15,0.43±0.06,0.37±0.04,0.83±0.11,respectively;the relative expression levels of Bax mRNA were1.00±0.12,2.13±0.25,1.96±0.21,1.38±0.17,respectively;the apoptosis rates were(100.00±13.55)%,(217.24±25.13)%,(196.37±21.53)%and(134.71±16.42)%,respectively.The above indicators of the experimental group were compared with the control group,and the above indicators of miR-373-3p mimics group were compared with NC group,and the differences were statistically significant(P<0.05,P<0.01,P<0.001).Conclusion Quercetin can induce apoptosis of breast cancer cells b
作者
李欢
温慧华
LI Huan;WEN Hui-hua(Department of Adult Critical Care Medicine,Inner Mongolia Autonomous Region Maternal and Child Health Care Hospital,Hohhot 010000,Inner Mongolia Autonomous Region,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2024年第18期2704-2708,共5页
The Chinese Journal of Clinical Pharmacology
