摘要
旨在了解四川省雷波县山羊和绵羊源寄生蜱种类及其携带斑点热群立克次体(spotted fever group Rickettsia,SFGR)和无形体(Anaplasmataceae)的流行情况。采集山羊和绵羊源寄生蜱,先进行形态学初步鉴定,然后提取蜱总DNA,分别扩增蜱ITS-2、立克次体gltA和ompA与无形体16S rRNA和rpoB的部分基因片段,并对所有PCR阳性产物测序、比对并构建遗传进化树,以确定蜱种类及其感染SFGR和无形体的情况。共采集到353只山羊和绵羊体表寄生蜱,经鉴定均为长角血蜱(Haemaphysalis longicornis)。SFGR总感染率为22.66%(80/353),仅检出1个种类R.raoultii,与西藏报道的银盾革蜱上分离的R.raoultii(JQ792163)亲缘关系最近。无形体总感染率为87.82%(310/353),共检出6种无形体和2种埃立克体,分别为A.bovis、A.capra、A.marginale、Anaplasma cf.marginale、Candidatus A.mediterraneum、Anaplasma sp.、Ehrichia chaffeensis和Ehrichia sp。部分长角血蜱存在混合感染,混合感染率为7.37%(26/353)。结果表明长角血蜱可能是当地的优势蜱种,SFGR和无形体的感染率较高,今后应加强上述蜱传病原的监测。
To investigate tick species and prevalence of Anaplasmataceae and spotted fever group Rickettsia(SFGR)in ticks collected from sheep and goats in Leibo county,Sichuan Province.Ticks were preliminary identified by morphological characters and the genomic DNAs of ticks were extracted.Molecular detection was performed by PCR targeting gene fragment of tick ITS-2,gltA and ompA genes of Rickettsia spp.,16S rRNA and rpoB genes of Anaplasma spp.and E-hrlichia spp.Positive PCR products were sequenced and phylogenetic trees were built based on these genes.The results showed that a total of 353 adult ticks collected from sheep and goats were identified as H.longicornis.The total prevalence of SFGR in H.longicornis was 22.66%(80/353)with only R.raoultii detected,which was closely related to R.raoultii(JQ792163)isolated from Dermacentor niveus in Tibet.The total prevalence of Anaplasmataceae was 87.82%(310/353)with six Ana plasta species(A.bovis,A.capra,A.marginale,Anaplasma cf.marg inale,Ana plasaa sp.,Candidatus A.mediterraneum)and two E-hrlichia species(E.chaf feensis and Ehrlichia sp.)detected.Co-infection was found in some H.longicornis as high as 7.37%(26/353).The results indicated that H.Iongicornis may be the dominant tick species in Leibo county with high infections rates of Anaplasmataceae and SFGR.The close monitoring of the tick-borne pathogens should be strengthened in the future research work.
作者
吉克日洪
向阳
陈虹汐
袁东波
尹念春
郝力力
JIKE Rihong;XIANG Yang;CHEN Hongxi;YUAN Dongbo;YIN Nianchun;HAO Lili(College of Animal Husbandry and Veterinary Medicine,Southwest Minzu University,Chengdu 610041,China;Yanting County Highend Talent Service Center,Sichuan 626100,China;Center for Animal Disease Control and Prevention in Sichuan Province,Chengdu 610041,China;Suining Agriculture and Rural Affairs Bureau,Suining,Sichuan 629000,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2024年第6期1194-1203,共10页
Chinese Journal of Veterinary Science
基金
西南民族大学研究生创新型科研资助项目(ZD2022668)。