摘要
[目的]探索茶黄素对氧化型低密度脂蛋白(ox-LDL)诱导的THP-1巨噬细胞泡沫化和氧化应激的影响及机制。[方法]使用50μmol/L茶黄素、10μmol/L核因子E2相关因子2(NRF2)抑制剂ML385预处理THP-1来源的巨噬细胞,随后加入100 mg/L ox-LDL刺激细胞24 h建立泡沫细胞模型。通过CCK-8法和LDH释放量检测茶黄素对THP-1巨噬细胞活力的影响。通过RT-qPCR和Western blot检测炎症因子白细胞介素6(IL-6)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)的表达;ELISA法检测炎症因子的释放。采用油红O染色检测细胞内脂质积累,DiL标记氧化型低密度脂蛋白(DiL-ox-LDL)染色检测脂质摄取,DCFH-DA探针检测活性氧(ROS)水平。通过Western blot和RT-qPCR检测脂质摄取、胆固醇外排及氧化应激相关蛋白的表达。[结果]经100 mg/L的ox-LDL处理,THP-1巨噬细胞活力明显降低,脂质摄取、积累明显增加,脂质摄取相关蛋白表达增加,胆固醇外排相关蛋白表达明显减少,炎症与ROS水平明显增加,髓过氧化物酶(MPO)和NADPH氧化酶2(NOX2)表达增加(P<0.05);加入茶黄素后,细胞活力升高,细胞内脂质积累明显减少,脂质摄取相关蛋白的表达明显减少,胆固醇外排相关蛋白表达明显增多,炎症因子IL-6、IL-1β、TNF-α的表达与释放明显降低,ROS水平降低,MPO与NOX2表达减少(P<0.05)。茶黄素预处理改变了NRF2信号通路中NRF2、血红素加氧酶1(HO-1)、Kelch样ECH关联蛋白1(KEAP1)的表达,增加了NRF2核易位,减缓了细胞内氧化应激。ML385预处理细胞后,NRF2、HO-1、KEAP1和CD36蛋白表达水平明显降低。[结论]茶黄素可以显著抑制THP-1巨噬细胞泡沫化,抑制ox-LDL诱导的THP-1巨噬细胞炎症并通过NRF2/HO-1信号通路减缓了氧化应激。
Aim To investigate the effect of theaflavin on oxidized low density lipoprotein(ox-LDL)-induced foam cell formation and oxidative stress in THP-1 macrophages and its mechanism.Methods THP-1 derived macrophages were pretreated with 50μmol/L theaflavin and(or)10μmol/L nuclear factor erythroid 2-related factor 2(NRF2)inhibitor ML385,then 100 mg/L ox-LDL was added to the cells for 24 h to establish the foam cell model.The effect of theaflavin on THP-1 macrophages viability was evaluated by CCK-8 assay and LDH release.The expression of inflammatory cytokines interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)were analyzed by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot.The release of inflammatory cytokines were detected by enzyme linked immunosorbent assay(ELISA).Intracellular lipid accumulation was detected by Oil red O staining,and lipid absorption was observed by DiL-labeled oxidized low density lipoprotein(DiL-ox-LDL)staining.Re-active oxygen species(ROS)level was detected by DCFH-DA probe.The expression of lipid uptake,cholesterol efflux and oxidative stress-related proteins were detected by Western blot and RT-qPCR.Results Treatment with 100 mg/L ox-LDL significantly decreased cell viability and cholesterol efflux-related protein expressions,increased lipid uptake,ac-cumulation and lipid uptake-related protein expressions,and significantly promoted inflammation and ROS level,as well as the expressions of myeloperoxidase(MPO),NADPH oxidase 2(NOX2)in THP-1 macrophages(all P<0.05).After pretreatment with theaflavin,cell viability was increased,intracellular lipid uptake,accumulation and lipid uptake-related protein expressions were significantly reduced,cholesterol efflux-related protein expressions were significantly increased,the expression and release of IL-6,IL-1βand TNF-αwere significantly decreased,ROS level was significantly decreased,and the expression of MPO and NOX2 were decreased(all P<0.05).Pretreatment with theaflavin effectively alleviated intr
作者
石萌萌
黄锐
黄子乐
胡军威
肖靖杰
刘艳红
武军驻
SHI Mengmeng;HUANG Rui;HUANG Zile;HU Junwei;XIAO Jingjie;LIU Yanhong;WU Junzhu(Taikang Medical School&School of Basic Medical Sciences,Wuhan University,Wuhan,Hubei 430071,China;Department of Cardiology,Zhongnan Hospital of Wuhan University,Wuhan,Hubei 430071,China;Department of Clinical Laboratory&Institute of Translational Medicine,Renmin Hospital of Wuhan University,Wuhan,Hubei 430060,China)
出处
《中国动脉硬化杂志》
CAS
2024年第9期747-755,共9页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金项目(82300322)。
