摘要
目的:评估卵巢微环境内邻苯二甲酸酯(phthalates,PAEs)暴露与炎性因子水平的关系。方法:纳入2020年4—12月接受体外受精/卵胞质内单精子注射-胚胎移植治疗的64例患者,测定其卵泡液内10种邻苯二甲酸酯代谢物(phthalate metabolites,mPAEs)及2种炎性因子的水平。通过多元线性回归模型评估卵巢微环境内mPAEs暴露与炎性因子水平的关系,并进一步按年龄分层分析。结果:卵泡液中邻苯二甲酸单丁酯(MBP)水平与肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平(β=0.446,P=0.003)及白细胞介素-6(interleukin-6,IL-6)水平(β=0.425,P=0.003)均呈正相关,邻苯二甲酸(2-乙基-5-氧己基)单酯(MEOHP)水平与TNF-α水平呈正相关(β=0.411,P=0.019)。在分层分析中,年龄≥30岁组卵泡液中MBP水平与TNF-α水平(β=0.667,P=0.000)及IL-6水平(β=0.407,P=0.028)均呈正相关,在年龄<30岁组内未观察到mPAEs与炎症因子之间存在关联。结论:PAEs暴露可影响卵泡液中炎性因子水平,提示PAEs暴露可能带来卵巢微环境炎症负荷的改变。
Objective:To assess the associations between phthalates(PAEs)exposure and the levels of inflammatory cytokines in the ovarian microenvironment.Methods:64 females who were undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer(IVF/ICSI-ET)from April to December in 2020 were included in this cross-sectional study.The levels of 10 kinds of phthalate metabolites(mPAEs)and 2 kinds factors in follicular fluid were measured.Multivariable linear regression model and further stratified analysis by age were conducted to analyze the associations between mPAEs and inflammatory factors.Results:There were significant positive associations between mono-butyl phthalate(MBP)and tumor necrosis factor-α(β=0.446,P=0.003)and interleukin-6(β=0.425,P=0.003)in the follicular fluid.Similarly,there was a significant positive relationship between mono(2-ethyl-5-oxohexyl)phthalate(MEOHP)and tumor necrosis factor-α(β=0.411,P=0.019).In the stratified analysis,MBP still showed significantly the positive association with tumor necrosis factor-α(β=0.667,P=0.000)and interleukin-6(β=0.407,P=0.028)in the group aged≥30 years.In contrast,there were no correlations between mPAEs and inflammatory factors within the group aged<30 years.Conclusions:Our results suggest that there is strongly association between PAEs exposure and the altered levels of inflammatory factor,and that PAEs exposure may bring changes of inflammatory burden in the ovarian microenvironment.
作者
肖楠
李永程
姚义鸣
孙红文
姚汝强
陈泳君
殷宇辰
罗海宁
XIAO Nan;LI Yong-cheng;YAO Yi-ming;SUN Hong-wen;YAO Ru-qiang;CHEN Yong-jun;YIN Yu-chen;LUO Hai-ning(Center for Reproductive Medicine,Tianjin Central Hospital of Gynecology Obstetrics,Tianjin Key Laboratory of Human Development and Reproductive Regulation,Tianjin 300100,China;College of Environmental Science and Engineering,Nankai University,Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria,Tianjin 300350,China;Clinical School of Obstetrics and Gynecology Center,Tianjin Medical University,Tianjin 300270,China)
出处
《国际生殖健康／计划生育杂志》
CAS
2024年第5期353-360,共8页
Journal of International Reproductive Health/Family Planning
基金
天津市科技计划项目(21JCZDJC00330)
天津市卫生健康科技项目(TJWJ2023MS028)
天津市医学重点学科(专科)建设项目(TJYXZDXK-043A)。
关键词
邻苯二醛类
甲酸酯类
环境污染物
卵泡液
肿瘤坏死因子α
白细胞介素6
体外受精
精子注射
细胞质内
Phthalic anhydrides
Formic acid esters
Environmental pollutants
Follicular fluid
Tumor necrosis factor-alpha
Interleukin-6
Fertilization in vitro
Sperm injections,intracytoplasmic
