摘要
针对蛋白酶难以降解高硫羊毛角蛋白的问题,构建了由底物激活剂LKZ-630与蛋白酶Savinase 16L组成的蛋白酶催化体系,采用该体系联合机械刮毯和刷毯对羊毛地毯进行丝光处理。通过酶活力、酶吸附性、羊毛水解度、地毯颜色、X射线衍射能谱及红外谱图等测试,分析了LKZ-630对酶水解羊毛的促进作用以及丝光处理前后羊毛地毯的外观及微观结构变化。结果表明:LKZ-630可打开羊毛纤维中二硫键,2 g/L的LKZ-630可使酶对羊毛的活力提高1575倍,可使羊毛纤维对酶的平衡吸附量提高12.7倍,使酶对羊毛的水解速率提高36.3倍;刮毯和刷毯作用使得处理后羊毛地毯中纤维细度由根部至尖端逐渐变细,处理后地毯触感柔软、有层次感且有蚕丝般光泽,其CIE亮度值增加6.99,白度值增加50.12,黄度值减小14.84。
Objective In order to fundamentally solve the severe environmental pollution problem caused by the existing chlorinated wool carpet mercerizing finishing,much research has been focused on ecological mercerizing.The protease method is a mild and green finishing process.However,the low hydrolysis efficiency of protease on wool keratin remains to be a technical bottleneck,and it is necessary to develop the protease method for the wool carpet mercerizing process with ecological characteristics.It is conducive to promoting green and sustainable development of the textile industry.Method A protease-catalyzed system consisting of substrate activator LKZ-630 and protease Savinase 16L was constructed and used for mercerizing wool carpets by combining mechanical scraping and brushing.The role of LKZ-630 in the enzymatic degradation of wool was studied by measuring the sulfhydryl content on the wool surface,protease activity,protease adsorption,and degree of hydrolysis of wool.The structure of wool carpets before and after treatment was examined by scanning electron microscopy,Fourier infrared spectroscopy,and X-ray diffraction energy spectroscopy.Results The results showed that LKZ-630 could open disulfide bonds in wool fibers and convert them to sulfhydryl groups.It facilitated the rapid degradation of high-sulfur wool tissues by proteases.By adding LKZ-630,the protease activity(substrate of wool fibers)increased by 1575 times when the concentration of LKZ-630 was 2 g/L.Under the treatment conditions of LKZ-6302 g/L,Savinase 16L 1 g/L,and 50℃,LKZ-630 increased the equilibrium adsorption of protease on wool fibers by 12.7 times and increased the hydrolysis rate by 36.3 times.Wool carpet mercerizing equipment with brushing and scraping functions was developed.A protease-catalyzed system(LKZ-6302 g/L and Savinase 16L 1 g/L)combined with brushing and scraping was used for mercerizing pure wool carpets at 50℃for 40 min.Brushing and scraping cause the mechanical force on the carpet fiber to gradually increase from the tail
作者
王乐
段志欣
姚金波
刘建勇
卢建军
WANG Le;DUAN Zhixin;YAO Jinbo;LIU Jianyong;LU Jianjun(College of Textile Engineering,Taiyuan University of Technology,Taiyuan,Shanxi 030600,China;State Key Laboratory of Clean and Efficient Coal Utilization,Taiyuan University of Technology,Taiyuan,Shanxi 030024,China;School of Textile Science and Engineering,Tiangong University,Tianjin 300387,China)
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2024年第9期129-136,共8页
Journal of Textile Research
基金
山西省基础研究计划项目(20210302124459)。
关键词
蛋白酶
羊毛
地毯
蛋白酶催化体系
丝光处理
底物激活
protease
wool
carpet
protease catalytic system
mercerization
substrate activation
