摘要
目的 探讨消瘀丸对小鼠胫骨骨折后瘀肿损伤的作用机制。方法 将小鼠随机分为假手术组、模型组、消瘀丸(XYW)低剂量组[2.0g/(kg·d)]、消瘀丸(XYW)高剂量组[4.0g/(kg·d)]和阳性药组[三七伤药片,国药准字Z61020090,0.33g, 0.4(g/kg·d)]。假手术组小鼠制造伤口并缝合,其余4组均进行小鼠胫骨骨折造模,于造模前7d至造模后5d对各给药组给予灌胃给药,假手术组、模型组给予等剂量蒸馏水灌胃,消瘀丸组用“消瘀丸水溶液”灌胃,阳性药组用三七伤药片水溶液灌胃。采用苏木精-伊红(hematoxylin-eosin, HE)染色检测胫骨骨折后瘀肿损伤的病理损伤,并测量其瘀肿损伤。酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)试剂盒检测各组小鼠化学趋化因子(MCP-1)、白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平,以及超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)以及还原型谷胱甘肽(GSH)。蛋白免疫印记(Western blot)技术检测小鼠肌肉组织中血红素氧合酶1(heme oxygenase1,HO-1)、醌氧化还原酶NADH 1[NAD(P)H quinone oxidoreductase 1,NQO1]、核转录因子E2相关因子2(nuclear transcription factor E2-related factor 2,Nrf2)、核因子-κB[(nuclear factor-kappa B)-p65,NF-κB p65]、p38丝裂原活化蛋白激酶(mitogen-activated protein kinase, p38MAPK)蛋白表达水平。通过16S rDNA测序检测小鼠肠道菌群的变化,进而挖掘出XYW治疗小鼠骨折后引起的瘀肿损伤潜在机制。结果 与模型组相比,XYW可明显改善小鼠瘀肿损伤程度;降低血清炎性因子水平;减轻氧化应激损伤。HE染色结果表明,XYW组小鼠瘀肿损伤肌肉组织中炎性浸润情况显著改善。Western blot结果显示,与模型组相比,XYW可显著升高小鼠瘀肿损伤肌肉组织中HO-1、NQO1、Nrf2蛋白表达,并显著降低NF-κBp65、p38MAPK蛋白表达。16S rDNA测序结果表明,XYW可以改善�
Objective Exploring the mechanism of Xiaoyu Pill on the injury of blood stasis and swelling after tibial fracture in mice.Methods Randomly divide the mice into a sham surgery group,a model group,a low-dose group of Xiaoyu Pill(XYW)[2.O g/kg:d],a high-dose group of Xiaoyu Pill(XYW)[4.O g/kg·d],and a positive drug group[Sanqi Shang Yao Pian,National Medical Standard Z61020090,0.33 g,0.4 g/kg·d].The sham group of mice made wounds and stitched them up,while the other four groups were used to create models of tibial fractures in mice.From 7 days before modeling to 5 days after modeling,each treatment group was given gastric gavage.The sham surgery group and model group were given equal doses of distilled water by gastric gavage,while the Xiaoyu Pill group was given"Xiaoyu Pill Water Solution"by gastric gavage.The positive drug group was given Sanqi Shangyao Tablet Water Solution by gastric gavage.Hematoxylin-eosin(HE)staining was used to detect pathological damage and measure stasis injury after tibial fracture.The enzyme-linked immunosorbent assay(ELISA)kit was used to detect the levels of chemotactic factor(MCP-1),interleukin-1β(IL-1p),tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD),malondialdehyde(malondialdehyde),and superoxide dismutase(superoxide dismutase)(SOD)in various groups of mice.α(TNF-α)levels,as well as superoxide dismutase(SOD),malondialdehyde(MDA),and reduced glutathione(GSH).Western blot(WB)technique was used to detect heme oxygenase 1(HO-1),quinone oxidoreductase NADH 1[NAD(P)H quinone oxidoreductase 1,NQO1],nuclear transcription factor E2-related factor 2(Nrf2),nuclear factor-B-p65(NF-Bp65)and p38 mitogen-activated protein kinase(p38-MAPK)protein expression levels in mouse muscle tissue.By using 16S rDNA sequencing to detect changes in mouse gut microbiota,potential mechanisms of stasis and swelling damage caused by XYW treatment of mouse fractures were explored.Results Compared with the model group,XYW significantly improved the degree of contusion and swelling injuries in mice,red
作者
牛驰程
曾平
刘金富
王伟伟
许青源
李豪
陶红成
李佳侣
丁强
郭良
NIU Chicheng;ZENG Ping;LIU Jinfu;WANG Weiwei;XU Qingyuan;LI Hao;TAO Hongcheng;LI Jialv;DING Qiang;GUO Liang(Guangri University of Chinese Medicine,Nanning 530200,China;The second Department of Orthopaedics,the First Affiliated Hospital of Guangri University of Chinese Medicine)
出处
《中国病原生物学杂志》
CSCD
北大核心
2024年第10期1147-1152,1157,共7页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.82160913)。
关键词
消瘀丸
胫骨骨折后瘀肿损伤
炎症
氧化应激
肠道菌群
Xiaoyu Pill
stasis and swelling injury after tibial fracture
inflammation
oxidative stress
intestinal microbiota
