摘要
目的了解泰州市预包装糕点中呕吐型蜡样芽胞杆菌腹泻型毒力基因、耐药性及分子分型情况。方法利用聚合酶链反应(PCR)方法对泰州市预包装糕点中97株蜡样芽胞杆菌分离株进行呕吐型菌株的鉴别,并检测呕吐型菌株是否携带致腹泻的毒力基因,用纸片扩散法对呕吐型菌株进行19种抗生素的敏感性测试,用多位点序列分型技术进行分子分型。结果97株蜡样芽胞杆菌中共检出5株呕吐型蜡样芽胞杆菌,分为ST 26和ST 1186两个型别;非溶血性肠毒素基因nhe、肠毒素基因entFM、bceT是5株菌株的主要腹泻型毒力基因;5株菌株对青霉素、氨苄西林、头孢噻肟完全耐药,对复方新诺明、利福平、头孢唑啉的耐药率分别为80%(4/5)、40%(2/5)、20%(1/5),对阿莫西林、克林霉素、头孢西丁的敏感率分别为40%(2/5)、80%(4/5)、80%(4/5),对其他抗生素则完全敏感。结论本研究显示泰州市预包装糕点中呕吐型蜡样芽胞杆菌的检出率为5.2%(5/97),以ST 26型为主要型别,携带多种腹泻型毒力基因。
Objective To understand the diarrhea-type virulence genes,the antimicrobial resistance and the molecular typing of emetic Bacillus cereus from the pre-packaged pastries in Taizhou City.Methods The Polymerase Chain Reaction(PCR)method was used to identify the vomiting strains of 97 Bacillus cereus strains which were isolated from prepackaged pastries in Taizhou and detecte whether the vomiting strains carry diarrhea virulence genes.The sensitivity of the vomiting strains to 19 antibiotics was tested by disk diffusion method,and the molecular typing was carried out by multilocus sequence typing technique.Results The results showed that five emetic Bacillus cereus stains were detected of 97 Bacillus cereus strains and divided into ST 26 and ST 1186 two types.Non-hemolytic enterotoxin gene nhe,enterotoxin genes entFM and bceT were the main diarrhea-type virulence genes of the five strains.The five strains were completely resistant to penicillin,ampicillin and cefotaxime.The resistance rates to cotrimoxazole,rifampicin and cefazolin were 80%(4/5),40%(2/5)and 20%(1/5),respectively.The sensitivity rates of amoxicillin,clindamycin and cefoxitin were 40%(2/5),80%(4/5)and 80%(4/5),respectively,and they were completely sensitive to other antibiotics.Conclusion This study showed that the detection rate of vomiting Bacillus cereus in pre-packaged pastries in Taizhou was 5.2%(5/97),ST 26 was the main type,there were a variety of diarrhea-type virulence genes in the five vomiting Bacillus cereus strains.
作者
王萍
陈琳
乔勇升
胡慧
董贵军
张占林
WANG Ping;CHEN Lin;QIAO Yongsheng;HU Hui;DONG Guijun;ZHANG Zhanlin(Taizhou Institute for Food Inspection,Jiangsu Provincial Key Laboratory of Market Supervision-Risk Identification and Key Analysis Technology of Food for Special Medical Purposes,Jiangsu Taizhou 225300,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2024年第6期653-658,共6页
Chinese Journal of Food Hygiene
基金
江苏省市场监督管理局科技计划(KJ204140)。
关键词
呕吐型蜡样芽胞杆菌
预包装糕点
毒力基因
耐药性
多位点序列分析
Emetic Bacillus cereus
pre-packaged pastry
virulence gene
antimicrobial resistance
multilocus sequence typing
