摘要
目的采用总量统计矩法分析补阳还五汤复方与单味药(中药饮片)挥发油指纹图谱,研究补阳还五汤中单味药-复方提取过程中质量的传递性和稳定性。方法分别采用水蒸气蒸馏法获取补阳还五汤复方及组方药材挥发油,建立气相色谱-质谱联用(GC-MS)指纹图谱,计算分析复方与单味药总量统计矩各参数的传递规律,并分析复方和单味药挥发性成分的变化。结果建立补阳还五汤GC-MS测定方法,获得组方药材和复方指纹图谱总量统计矩参数。复方挥发油图谱得到65个成分,其中45个化合物来自组方药材挥发油。药材至复方总体挥发性成分的传递性为88.74%,说明药材及复方在成分和含量上遵循Hard-Weinberg平衡,也验证了指纹图谱总量统计矩的加合性。结论该方法为补阳还五汤质量控制提供了方法支撑,统计矩的加合性能阐述药材-复方的传递性,旨为经典名方质量控制方面提供一种新思路。
Objective To analyze the fingerprint of the volatile oil of compound and single medicinal materials in Buyang Huanwu Decoction(BYHWD)(decoction pieces)by total statistical moment method,and to study the transferability and stability of single and compound medicinal materials in BYHWD extraction process.Methods The volatile oils of BYHWD and its components were obtained by steam distillation,and the gas chromatography-mass spectrometry(GC-MS)fingerprints were established.The transferring rules of the parameters of the total statistical moment of the compound and single medicinal materials were calculated and analyzed,and the changes in the volatile components of the compound and single medicinal materials were analyzed.Results The GC-MS determination method of BYHWD was established,and the statistical moment parameters of the total amount of the fingerprint of the single and the compound medicinal materials were obtained.Sixty five components were obtained from the volatile oil of the compound,of which 45 compounds were from the volatile oil of the compound medicinal materials.The transferability of the total volatile components from single medicinal materials to the compound medicinal materials was 88.74%,indicating that the single medicinal materials and the compound medicinal materials followed the Hard-Weinberg equilibrium in terms of composition and content,and verified the additivity of the total statistical moment of the fingerprint.Conclusion The research has provided methodological support to the quality control of BYHWD.The additive performance of statistical moment has expounded the transitivity of single medicinal materials to compound medicinal materials,aiming to offer a new idea for the quality control of classical renowned formulas.
作者
李文姣
唐昱
贺福元
潘雪
LI Wenjiao;TANG Yu;HE Fuyuan;PAN Xue(College of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Hunan Key Laboratory of Pharmaceutics and Preparation of Chinese Medicine,Changsha,Hunan 410208,China;Supramolecular Mechanism and Mathematical Characterization Laboratory of Chinese Medicine,Hunan University ofChinese Medicine,Changsha,Hunan 410208,China)
出处
《湖南中医药大学学报》
CAS
2024年第9期1608-1613,共6页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金面上项目(81874507)
湖南省自然科学基金面上项目(2022JJ30453)
湖南中医药大学校级科研项目(2022XJZKC006)。
关键词
补阳还五汤
指纹图谱
挥发油
总量统计矩
传递性
Buyang Huanwu Decoction
fingerprint
volatile oil
total statistical moment
transferability
