摘要
以废弃烟叶为来源,分离筛选产细菌纤维素菌株,对筛选菌株进行形态学观察、生理生化试验及分子生物学鉴定。以废弃烟叶浸提液为碳源和氮源,采用筛选菌株与酿酒酵母(Saccharomyces cerevisiae)以混菌(1∶1)发酵,考察废弃烟叶浸提液添加量、混菌接种量、pH、发酵温度及发酵时间对细菌纤维素产量的影响,并利用单因素、Plackett-Burman和Box-Benhnken试验优化混菌发酵工艺条件。结果表明,筛选得到一株产细菌纤维素菌株YC1,其被鉴定为氧化葡萄糖酸杆菌(Gluconobacter oxydans)。最佳混菌发酵工艺条件为:废弃烟叶浸提液添加量10%,菌株YC1与酿酒酵母(1∶1)混菌接种量1.0%,初始pH值5.5,发酵温度30℃,发酵时间6 d。在该优化条件下,细菌纤维素产量最大,为2.71 g/L,比菌株YC1单独发酵细菌纤维素产量(1.18 g/L)提高了1.29倍。
The bacterial cellulose-producing strains were isolated and screened from waste tobacco leaves,and the strains were identified by morphologic observation,physiological and biochemical tests and molecular biological identification.Using waste tobacco extract as carbon and nitrogen source,the mixed-strains fermentation was carried out with screened strain and Saccharomyces cerevisiae(1:1),and the effects of waste tobacco extract addition,mixed-strains inoculum,pH,fermentation temperature and time on the yield of bacterial cellulose were investigated.The results showed that the fermentation process conditions were optimized by single factor,Plackett-Burman and Box-Behnken experiments.The results showed that a bacterial cellulose-producing strain YC1 was selected and identified as.The optimal mixed-strains fermentation process conditions were as follows:waste tobacco extract addition 10%,strain YC1 and Gluconobacter oxydans(1:1)inoculum 1.0%,initial pH 5.5,fermentation temperature 30益and time 6 d.Under these optimal process conditions,the bacterial cellulose yield was the highest(2.71 g/L),which was 1.29 times higher than that of strain YC1 fermentation alone(1.18 g/L).
作者
黄蕾
李媛媛
何爱民
陈伟华
王晓如
崔冰
来苗
赵铭钦
HUANG Lei;LI Yuanyuan;HE Aimin;CHEN Weihua;WANG Xiaoru;CUI Bing;LAI Miao;ZHAO Mingqin(College of Tobacco Science,Henan Agricultural University,Zhengzhou 450046,China;China Tobacco Hebei Industrial Co.,Ltd,Shijiazhuang 05005l,China)
出处
《中国酿造》
CAS
北大核心
2024年第9期92-98,共7页
China Brewing
基金
河北中烟工业有限责任公司重点科技项目(20231300003400038)。
关键词
产细菌纤维素菌株
筛选鉴定
废弃烟叶
混菌发酵工艺
响应面优化
bacterial cellulose-producing strains
screening and identification
waste tobacco leaves
mixed-strains fermentation process
response surface optimization
