摘要
目的探讨血清微小RNA-138-5p(miR-138-5p)、Sorbin和SH3结构域连接蛋白2(SORBS2)水平与老年冠状动脉粥样硬化性心脏病(冠心病)患者斑块稳定性及病变程度的相关性。方法取2022年7月至2024年1月于石家庄市人民医院收治的131例老年冠心病患者,根据超声结果确定血管腔内的斑块情况分为稳定组52例和不稳定组79例;根据冠状动脉造影结果进行Gensini评分,结合病变范围分为单支病变组42例,双支病变组46例和三支病变组43例。另外选取同期在本院进行体检的49例健康者作为对照组。采用实时荧光定量聚合酶链反应(qRT-PCR)对两组血清miR-138-5p和SORBS2水平检测,采用Pearson法和Spearman法分析血清miR-138-5p和SORBS2水平与老年冠心病患者斑块稳定性及病变程度相关性。结果不稳定组血清miR-138-5p低于稳定组、对照组,且稳定组低于对照组。不稳定组血清SORBS2高于稳定组和对照组,且稳定组高于对照组(P<0.05)。Pearson法分析结果显示,老年冠心病患者血清miR-138-5p水平与SORBS2水平呈负相关(P<0.05)。高密度脂蛋白胆固醇(HDL-C)、血清miR-138-5p水平是老年冠心病患者不稳定斑块形成的保护因素,三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、血清SORBS2水平是老年冠心病患者不稳定斑块形成的危险因素(P<0.05)。与单支病变组相比,双支病变组、三支病变组患者血清miR-138-5p水平降低,其中三支病变组降低更显著(P<0.05),血清SORBS2水平、Gensini评分升高,其中三支病变组升高更多(P<0.05)。Spearman相关性分析结果显示,老年冠心病患者血清miR-138-5p水平与Gensini评分呈负相关,血清SORBS2水平与Gensini评分呈正相关。结论血清miR-138-5p和SORBS2水平与老年冠心病患者斑块稳定性及病变程度密切相关。
Objective To investigate the correlation among levels of serum microRNA-138-5p(miR-138-5p)andSorbin and SH3 domain-containing protein 2(SORS2),plaque stability and lesion severity in elderly patients with coronary heart disease(CHD).Methods Elderly CHD patients(n=131)were chosen and divided,according to plaque situation determined by ultrasound results,into stable group(n=52)and unstable group(n=79)from July 2022 and Jan.2024.According to results of coronary angiography(CAG),Gensini scores andlesion extent,the patients were divided into single-vessel lesion group(n=42),double-vessel lesion group(n=46)and triple-vessel lesion group(n=43).In addition,healthy controls(n=49)were chosen into control group.The levels of serum miR-138-5p and SORBS2 were detected by using qRT-PCR,and correlation among miR-138-5p,SORBS2,plaque stability and lesion severity were analyzed by using Pearson method and Spearman method.Results The level of miR-138-5p was lower and level of ORBS2 was higher in unstable group than those in stable group and control group,which were more significant in stable group(P<0.05).The results of Pearson method showed that miR-138-5p level was negatively correlated to SORBS2 level(P<0.05).The levels of high-density lipoprotein-cholesterol(HDL-C)and miR-138-5p level were protective factors for unstable plaque formation,and levels of triglyceride(TG),low-density lipoprotein-cholesterol(LDL-C)and SORBS2 level were risk factors for unstable plaque formation(P<0.05)in elderly CHD patients.The level of miR-138-5p decreased,and SORBS2 level and Gensini scores increased in double-vessel lesion group and triple-vessel lesion group compared with single-vessel lesion group,which were more significantly in triple-vessel lesion group(P<0.05,P<0.05).The results of Spearman correlation analysis showed that miR-138-5p level was negatively correlated to Gensini scores,and SORBS2 level was positively correlated to Gensini scores.Conclusion The levels of serum miR-138-5p and SORS2 are closely correlated to plaque stability an
作者
高阿芳
郑梅
胡钦
Gao Afang;Zheng Mei;Hu Qin(Department of Cardiology,People's Hospital of Shijiazhuang City,Shijiazhuang 050001,China;不详)
出处
《中国循证心血管医学杂志》
2024年第7期818-821,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
河北省医学科学研究课题(20190873)。
