摘要
目的:基于银杏转录组深度测序,通过构建原核表达系统工程菌株,系统研究银杏中萜类合成酶的功能。方法:根据蛋白功能的预测,利用不同的原核表达体系进行功能表征,GC-MS检测分析。结果:银杏GbTPS3基因可以同时表达两个单萜化合物(龙脑和2-蒎烷醇)。结论:银杏GbTPS3为Class I型萜类合成酶,主要负责参与萜类化合物起始的合成反应。其特点是碳骨架重排的随机性可以产生不同的碳正离子中间体,在底物唯一的情况下,单一萜类合成酶往往会产生多种产物。
Objective:Based on deep transcriptome sequencing of Ginkgo biloba,a prokaryotic expression system engineering strain was constructed to systematically study the function of terpenoid synthase in Ginkgo biloba.Methods:Based on the prediction of protein function,different prokaryotic expression systems were used for functional characterization and GC-MS detection analysis.Results:The GbTPS3 gene of Ginkgo biloba can simultaneously express two monoterpenoids(borneol and 2-pinanol).Conclusion:Ginkgo biloba GbTPS3 is Class I terpenoid synthase enzymes,mainly responsible for participating in the synthesis reaction of terpenoid compounds.Its characteristic is that the randomness of carbon skeleton rearrangement can produce different carbocation intermediates.In the case of a unique substrate,a single terpenoid synthase often produces multiple products.
作者
张文锦
姜海晶
吴翀
卢华卫
Zhang Wenjin;Jiang Haijing;Wu Chong;Lu Huawei(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《山东化工》
CAS
2024年第16期36-39,43,共5页
Shandong Chemical Industry
基金
贵州中医药大学博士启动基金。
关键词
银杏
萜类合成酶
萜类化合物
催化机制
功能表征
Ginkgo biloba
terpene synthase
terpene compounds
catalytic mechanism
functional characterization
