摘要
为探索FAST周边喀斯特峰丛洼地不同植被演替阶段土壤微生物群落结构变化规律及其影响因素,该文以FAST周边草本、灌木、乔木3个演替阶段表层土壤为研究对象,采用高通量测序技术,研究不同演替阶段土壤微生物多样性、群落组成及与土壤化学性状之间的关系。研究发现:随着演替进行,土壤细菌和真菌的Shannon指数和Chao1指数呈显著下降趋势。不同演替阶段变形菌、放线菌和酸杆菌均为优势细菌门,分别占细菌群落的34.83%、19.22%、18.56%;子囊菌和担子菌为优势真菌门,分别占真菌群落的40.72%、40.11%。Pearson相关性分析发现,土壤pH、有机质、全氮、碱解氮和有机质显著影响细菌和真菌多样性;Spearman相关性分析发现,全磷、全钾、速效钾、氧化铝和交换性镁与变形菌门呈显著负相关关系(P<0.05),土壤pH、交换性钙与放线菌门呈显著正相关关系(P<0.01),铁铝氧化物与子囊菌门呈显著负相关关系(P<0.01),有效磷与担子菌门呈显著负相关关系(P<0.01)。冗余分析和Mantel分析表明,土壤pH是影响细菌和真菌群落组成的共有关键化学因子。研究结果对深入理解峰丛洼地喀斯特森林土壤微生物的变化特征与规律、科学保护和发展FAST周边喀斯特森林生态环境具有重要参考价值。
To explore the changes in soil microbial community structure and its influencing factors in different vegetation succession stages of karst peak-valley landscapes around FAST,this study took the surface soil of the three vegetation succession stages of herbaceous,shrubby,and woody vegetation as research objects,and used high-throughput sequencing technology to study the diversity,community composition,and relationships with soil chemical properties of soil microorgan-isms.The study found that as the succession progressed,the Shannon index and Chao1 index of soil bacteria and fungi showed a significant downward trend.The actinobacteria,actinomyces,and acidimicrobia were all dominant bacterial phyla in differ-ent succession stages,accounting for 34.83%,19.22%,and 18.56%of the bacterial community,respectively.The ascomycetes and basidiomycetes were dominant fungal phyla,accounting for 40.72%and 40.11%of the fungal community,respectively.According to the Pearson correlation analysis,soil pH,organic matter,total nitrogen,ammonium nitrogen,and organic matter significantly affected bacterial and fungal diversity.According to the Spearman correlation analysis,total phosphorus,total potassium,available potassium,aluminum oxide,and exchangeable magnesium were significantly negatively correlated with the proteobacteria phylum(P<0.05),soil pH and exchangeable calcium were significantly positively correlated with the actino-myces phylum(P<0.01),ferrimagnetic oxides were significantly negatively correlated with the ascomycetes phylum(P<0.01),and available phosphorus was significantly negatively correlated with the basidiomycetes phylum(P<0.01).Redundancy anal-ysis and Mantel analysis collectively indicate that soil pH is a key chemical factor that influences the composition of bacterial and fungal communities in the karst forest.The above results have important reference value for understanding the characteris-tics and laws of changes in the soil microbial community in the peak forest karst area,scientific development,and prot
作者
侯超
张成富
张孙健
曹洋
蔡国俊
张建利
苏维词
张丽敏
HOU Chao;ZHANG Chengfu;ZHANG Sunjian;CAO Yang;CAI Guojun;ZHANG Jianli;SU Weici;ZHANG Limin(College of Ecological and Environmental Engineering,Guizhou Minzu University,Guiyang 550025,China;Guizhou Institute of Mountain Resources,Guiyang 550001,China;Guizhou Institute of Analysis and Testing,Guiyang 550013,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2024年第8期174-185,共12页
Environmental Science & Technology
基金
贵州省科技计划项目(黔科合基础-ZK[2024]重点092)
国家自然科学基金(42161052)
贵州省科技支撑计划(黔科合支撑[2022]一般200)
贵州省科技支撑项目(黔科合支撑[2021]一般460)
贵州科学院博士基金(黔科院科专合字[2023]03号)。
关键词
FAST
峰丛洼地
高通量测序
土壤微生物
演替阶段
FAST
peak cluster depression
high-throughput sequencing
soil microorganism
successional stage
