摘要
【目的】筛选若尔盖高寒草地高产胞外多糖PGPR,为研制适宜若尔盖高寒草地的多糖菌剂及相关产品提供优良菌种资源。【方法】在16℃条件下,利用选择性培养基从高寒草地的波伐早熟禾(Poa poophagorum)和老芒麦(Elymus sibiricus)根际筛选高产胞外多糖PGPR,利用16S rDNA测序与系统发育分析明确分类地位,通过接种试验研究菌株和胞外多糖的促生特性。【结果】从两种植物根际筛选到3株产胞外多糖PGPR,其中,菌株NAHP4、PBRS1高产胞外多糖,产糖量分别为1 462.50、1 337.50 mg/L,并兼具固氮、溶无机磷的促生特性;胞外多糖对老芒麦的种子萌发和胚芽生长具有促进作用,高浓度的胞外多糖对胚根生长具有显著抑制作用(P<0.05);接种菌株NAHP4、NBHP13、PBRS1对老芒麦地下部分具有显著促进作用(P<0.05),其中,菌株NAHP4的促进作用最大。【结论】菌株NAHP4作为高产胞外多糖PGPR资源,具有研发适宜若尔盖高寒草地的多糖菌剂及相关产品的潜力。
【Objective】To screen high exopolysaccharide-producing PGPR resources from the Zoige alpine grassland in order to provide valuable strains for developing Polysaccharide bacterial reagents suitable for Zoige alpine grassland and related products.【Method】At 16℃,high exopolysaccharide-producing PGPR was isolatedf rom the rhizosphere of Poa poophagorum and Elymus sibiricus in alpine grassland using selective media.Their taxonomic sta-tus was determined by 16S rDNA sequencing and phylogenetic analysis.The growth-promoting characteristics of the strain and exopolysaccharide were evaluated through inoculation tests.【Result】Three strains of exopolysaccha-rides-producing PGPR were identified from the rhizosphere of the two plants.Among them,strains NAHP4 and PBRS1 were notable for their high exopolysaccharides production,with levels of 1462.50 and 1337.50 mg/L,re-spectively.This strains exhibited growth-promoting properties,including nitrogen fixation and inorganic phosphorus solulisation.Exopolysaccharides promoted seed germination and seedling growth of Elymus sibiricus,through high concentration of exopolysaccharides significantly inhibited radicle growth(P<0.05).The inoculated strains NAHP4,NBHP13 and PBRS1 lypromotedroot development in Elymus sibiricus(P<0.05),with NAHP4 showing-the greatest effect.【Conclusion】Strain NAHP4,as a high exopolysaccharide-producing PGPR resource,has signifi-cant potential for developing polysaccharide bacterial reagents and related products suitable for the alpine grassland of Zoige.
作者
王振龙
姚拓
李智燕
柴加丽
何礼
陈鑫
WANG Zhen-long;YAO Tuo;LI Zhi-lan;CHAI Jia-li;HE Li;CHEN Xin(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China;Grassland Technology Extension Station of Gansu Province,Lanzhou 730000,China)
出处
《草原与草坪》
CAS
CSCD
2024年第4期94-102,共9页
Grassland and Turf
基金
甘肃省优秀研究生“创新之星”项目(2021CXZX-386)
禾本科牧草复合菌肥应用与推广技术(KJCX2021010)。
关键词
植物根际促生菌
胞外多糖
高寒草地
多糖菌剂
plant
rhizosphere growth-promoting bacteria
exopolysaccharide
alpine grassland
polysaccharide bacterial reagent
