摘要
目的基于线粒体自噬PINK1/Parkin信号通路对比枳实、麸炒枳实和烫枳实对功能性消化不良(FD)大鼠的治疗作用。方法将36只大鼠随机分为正常组、模型组、多潘立酮组、枳实组、麸炒枳实组、烫枳实组。除正常组外其他各组采用碘乙酰胺灌胃结合夹尾刺激法制作FD大鼠模型。造模结束后各给药组给予相应药液灌胃,模型组和正常组给予等体积生理盐水灌胃,持续14 d。造模和给药时,记录大鼠饮食量、饮水量和体质量。给药结束后,测定大鼠小肠推进率,苏木-伊红(HE)染色观察胃组织损伤情况,Western blot检测自噬相关蛋白PINK1、Parkin、Beclin-1、p62的表达。结果与模型组比较,各给药组小肠推进率提高。在造模期间,与正常组比较,各组大鼠的体质量增长减慢,平均日饮食量降低,模型组大鼠平均日饮水量降低。在给药期间,与模型组比较,多潘立酮组、麸炒枳实组和烫枳实组平均日饮食量增加;与模型组比较,麸炒枳实组PINK1、Parkin蛋白表达增加,烫枳实组p62蛋白表达提高、Beclin-1蛋白表达降低,枳实组Beclin-1蛋白表达降低。结论枳实、麸炒枳实和烫枳实治疗FD的作用机制可能与抑制PINK1/Parkin信号通路激活有关,麸炒枳实对FD大鼠的治疗效果优于枳实和烫枳实。
Objective To investigate the therapeutic effects of Aurantii Fructus Immaturus(AFI),bran stir-fried Aurantii Fructus Immaturus and sand-heated Aurantii Fructus Immaturus on functional dyspepsia based on the mitophagy PINK1/Parkin signaling pathway.Methods 36 rats were randomly divided into normal group,model group,domperidone group,AFI group,bran stir-fried AFI group and sand-heated AFI group.In addition to the normal group,the FD rat model was made by iodoacetamide gavage combined with tail clamp stimulation in other groups.After the end of modeling,each drug group was given the corresponding liquid medicine by gavage,while the model group and the normal group were given the same volume of normal saline by gavage for 14 days.During modeling and administration,the diet,water intake and body weight of rats were recorded.After the end of administration,the intestinal propulsion rate of rats was measured.The damage of gastric tissue was observed by HE staining.The expression of autophagy-related proteins PINK1,Parkin,Beclin-1 and p62 were detected by Western blot.Results Compared with the model group,the intestinal propulsion rate of each administration group were increased.During the modeling period,compared with the normal group,the weight gain of rats in each group were slowed down,the average daily diet decreased,and the average daily water intake of rats in the model group decreased.During the administration period,compared with the model group,the average daily diet of the domperidone group,the bran stir-fried AFI group and the scalded AFI group increased.Compared with the model group,the expression of PINK1 and Parkin protein increased in the bran stir-fried AFI group,the expression of p62 protein were increased and the expression of Beclin-1 protein decreased in the sand-heated AFI group,and the expression of Beclin-1 protein decreased in the AFI group.Conclusion The mechanism of AFI,bran stir-fried AFI and sand-heated AFI in the treatment of functional dyspepsia may be related to the inhibition of PINK1/Par
作者
刘泽
张硕
徐欣雨
赵瑞华
罗容
LIU Ze;ZHANG Shuo;XU Xinyu;ZHAO Ruihua;LUO Rong(School of Chinese Medicine,Capital Medical University,Beijing 100069,China)
出处
《北京中医药》
2024年第8期880-885,共6页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81973431)
北京中医药薪火传承“新3+3”工程首批代表性成果项目(2023-DBXCG-05)。
关键词
枳实
麸炒枳实
烫枳实
功能性消化不良
线粒体自噬
PINK1/Parkin信号通路
Aurantii Fructus Immaturus
bran stir-fried Aurantii Fructus Immaturus
sand-heated Aurantii Fructus Immaturus
functional dyspepsia
mitochondrial autophagy
PINK1/Parkin signaling pathway
