摘要
【目的】验证中国柑橘属DUS测试指南中数量性状对柚类种质资源的适应性,为更客观更科学地描述柚类数量性状和修订柑橘属DUS测试指南提供参考。【方法】以中国柑橘属测试指南为标准,对117份柚类种质资源的25个数量性状进行数据采集,根据各性状的特点选择合适的分级方法并确定分级范围。【结果】25个数量性状品种内变异系数在2.96%~26.96%之间,品种间变异系数在10.33%~62.90%之间,大部分数量性状数据的多态性和稳定性较好;根据正态性检验结果对25个数量性状进行分级,得到了25个数量性状的分级标准,与柑橘属测试指南的分级数相比:花萼直径、果形指数、汁胞长度由3级增为5级,花瓣长度、花瓣宽度、叶片长度、叶片宽度、果皮厚度由9级减为7级,花瓣指数、叶形指数、中心柱大小、囊瓣数、果汁含量、可溶性固形物含量由9级减为5级,对果实质量、果实纵径和果实横径3个性状分别制定了柚和葡萄柚的分级标准;该分级标准下25个数量性状的多样性指数变化范围为0.878~1.954,性状间的相似系数分布在-0.69~0.91之间;通过主成分分析得到6个主成分,累积贡献率达72.76%。【结论】初步建立了柚类种质资源25个数量性状的分级指标,对柚类品种的性状评价、利用及良种选育具有一定指导意义,同时为中国柚类种质鉴定和DUS测试指南修订提供参考。
【Objective】The DUS(Distinctness,Uniformity and Stability)test refers to the process of testing the specificity,consistency and stability of new plant varieties.The DUS test guideline is the technical basis and standard for DUS test of tested varieties,and has become the technical basis for variety approval,variety registration and variety protection in China.At present,the DUS testing guideline of Citrus in China(NY/T 2435—2013)covers the entire Citrus genus.However,there are great differences in some characteristics between the different species in the same genus.Therefore,this study aimed to explore the adaptability of quantitative characteristics in the DUS testing guidelines for Citrus in China to pomelo varieties,and to provide reference for more objective and scientific description of quantitative characteristics of pomelo and revision of DUS test guidelines of Citrus.【Methods】In this study,twenty-five quantitative characteristics of the 117 pomelo germplasm resources in the National Citrus Germplasm Resource collection were observed according to the DUS testing guideline of Citrus in China.The data of these twenty-five quantitative characteristics were collected by measurement method.The normality of the data was comprehensively judged according to the significant test value of the K-S test for each quantitative characteristics,the fitting of the frequency distribution diagram and the normality curve,and the deviation between the measured value of the Q-Q diagram and the predicted normal value.The least significant difference method was used to grade the quantitative characteristics that conformed to the normal distribution,and the range method was used to grade the quantitative characteristics that did not conform to the normal distribution.【Results】The intra-variety variation coefficient of the twenty-five quantitative characteristics was between 2.96%and 26.96%,and the intervariety variation coefficient was between 10.33%and 62.90%.Most quantitative characteristics showed great polymorphis
作者
陈璞
徐振江
江东
易静
苏穆清
邓超
饶得花
CHEN Pu;XU Zhenjiang;JIANG Dong;YI Jing;SU Muqing;DENG Chao;RAO Dehua(College of Agriculture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Citrus Research Institute of Southwest University,Chongqing 400712,China;Development Center of Science and Technonlogy,Ministry of Agriculture and Rural Affairs,Beijing 100122,China)
出处
《果树学报》
CAS
CSCD
北大核心
2024年第9期1885-1902,共18页
Journal of Fruit Science
基金
农业农村部物种品种资源保护项目:申请保护品种DUS测试及已知品种库维护(h20230603)。
关键词
柚类
DUS测试
数量性状
分级
Pomelo
DUS testing
Quantitative characteristics
Grade
