摘要
菊科(Asteraceae)是真双子叶植物的一大类,具有复杂的头状花序,目前对其花发育的分子调控机制还知之甚少。APETALA1(AP1)属于MADS-box基因家族,在植物成花诱导和花器官发育中具有关键作用。本研究对欧洲千里光(Senecio vulgaris)AP1同源基因SvAP1-5进行了生物信息学及组织特异性表达分析,并基于VIGS技术创建了SvAP1-5基因沉默植株,并在龙葵(Solanum nigrum)中过表达SvAP1-5,对其功能进行了初步探讨。生物信息学分析结果表明,SvAP1-5基因具有典型的MADS-box和K-box结构,C末端含有FUL基序和paleoAP1基序,属于euFUL分支的AP1类基因。qRT-PCR结果表明,SvAP1-5在苞片、花瓣和心皮中均有表达,其中在心皮内较高表达。与对照相比,SvAP1-5基因沉默导致花瓣不规则裂开,柱头分裂增加,心皮发育不良。而SvAP1-5过表达龙葵植株的果实发育异常,出现类似果实细胞的增生组织。综上所述,在欧洲千里光花发育过程中,SvAP1-5基因可能参与花瓣和心皮的发育,并发挥着重要作用。
Asteraceae is a large class of eudicots with complex capitulum,and little is known regarding the molecular regulation mechanism of flower development.APETALA1(AP1)belongs to the MADS-box gene family and plays a key role in plant floral induction and floral organ development.In this study,the bioinformatics and tissue-specific expression of AP1 homologous gene SvAP1-5 in Senecio vulgaris were analyzed.Based on VIGS technology,SvAP1-5 gene silencing plants were created,and SvAP1-5 was overexpressed in Solanum nigrum.The results of bioinformatics analysis showed that SvAP1-5 gene had typical MADS-box and K-box structure,and contains FUL motif and paleoAP1 motif at the C-terminal.SvAP1-5 belongs to the euFUL branch of AP1 gene.qRT-PCR results showed that SvAP1-5 was expressed in bracts,petals and carpels,and was highly expressed in carpels.Compared with the control group,SvAP1-5 gene silencing resulted in irregular petal dehiscence,increased stigma division,and carpel dysplasia.The fruit development of SvAP1-5 overexpressing S.nigrum plants was abnormal,and the hyperplastic tissue similar to fruit appeared.In summary,SvAP1-5 gene may be involved in the development of petals and carpels and plays an important role during the development of S.vulgaris.
作者
张玉娜
郝燕敏
崔敏龙
朴春兰
Yuna Zhang;Yanmin Hao;Minlong Cui;Chunlan Piao(Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agriculture and Rural Affairs,Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province,College of Horticultural Science,Zhejiang A&F University,Hangzhou 311300,China)
出处
《遗传》
CAS
CSCD
北大核心
2024年第9期727-736,共10页
Hereditas（Beijing)
基金
浙江省农业科学院合作研发项目(编号:H20220312)资助。
