摘要
目的研究跨膜蛋白106B(transmembrane protein 106B,TMEM106B)支持新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)多种Omicron亚变异株感染侵入的特性。方法构建TMEM106B-knockout Flp-In T-Rex 293细胞系(TMEM106BKO),利用假病毒感染系统以及GFP1-10和GFP11的双荧光互补系统的合胞体形成实验检测TMEM106B在SARS-CoV-2多种Omicron亚变异株刺突蛋白(spike protein,S)介导的侵入和细胞-细胞融合中发挥的作用。结果SARS-CoV-2多种Omicron亚变异株对TMEM106B-knockout Flp-In T-Rex 293细胞的侵入效率存在不同程度的降低,并且TMEM106B可促进SARS-CoV-2多种Omicron亚变异株S蛋白介导的细胞-细胞融合。结论TMEM106B可作为受体分子支持SARS-CoV-2多种Omicron亚变异株的侵入和细胞-细胞融合,为治疗新型冠状病毒感染提供新的思路。
ObjectiveTo investigate the role of transmembrane protein 106B(TMEM106B)in supporting the entry of multiple Omicron subvariants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).MethodsTMEM106B-knockout Flp-In T-Rex293 cell line(TMEM106B KO)was constructed.The pseudoviral infection systems and the syncytia formation assay based on dual fluorescent complementary system of GFP1-10 and GFP11 were established to detect the role of TMEM106B in spike protein-mediated entry and cell-cell fusion during the infections by different SARS-CoV-2 Omicron subvariants.ResultsThe SARS-CoV-2 Omicron subvariants showed varying degrees of reduced efficiency in infecting TMEM106B KO,and TMEM106B can facilitate cell-cell fusion mediated by spike protein of various SARS-CoV-2 Omicron subvariants.ConclusionsTMEM106B acted as a receptor to support the entry and cell-cell fusion of multiple Omicron subvariants of SARS-CoV-2.It could be a new idea for the treatment of SARS-CoV-2 infection.
作者
王媛媛
陈丹瑛
刘雨欣
宋焱君
孙慧
张媛媛
赵学森
Wang Yuanyuan;Chen DanyingLiu Yuxin;Song Yanjun;Sun Hui;Zhang Yuanyuan;Zhao Xuesen(Peking University Ditan Teaching Hospital,Beijing 100015,China;Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China)
出处
《国际病毒学杂志》
北大核心
2024年第4期291-295,共5页
International Journal of Virology
基金
国家重点研发计划(2023YFC0872400)
北京市自然科学基金(M23005)。
关键词
跨膜蛋白106B
新型冠状病毒
Omicron亚变异株
Transmembrane protein 106B
Severe acute respiratory syndrome coronavirus 2
Omicron subvariantm
