摘要
目的注射内毒素导致犬发生脓毒症进而形成多脏器功能衰竭,构建犬的多器官功能障碍综合征(MODS)模型,通过研究肾脏的相关指标,进一步观察MODS犬的肾脏损伤变化,更好的了解MODS犬的肾脏损伤的发生机制,为临床和实验研究提供理论依据。方法14只家犬分为两组,其中MODS模型犬7只(实验组),正常犬7只(对照组)。通过分时段测量肾脏指标进一步了解肾脏损伤的情况。结果与对照组相比,实验组犬尿素氮和肌酐均在实验开始后2 h呈现进行性增高(P<0.05),尿量明显减少(P<0.05),出现多尿期的时间明显延后。病理结果表明脓毒症会导致肾小管发生损伤,但并未发生弥漫性坏死。结论实验犬在造模成功后2 h肾脏指标开始升高,肾脏的确发生了急性损伤,通过病理学也证明了肾脏的损伤,但肾小管并未发生弥漫性坏死。如果想挽救实验犬的肾脏功能需要在2 h前积极寻找引起肾脏损伤的原因,积极治疗原发病,为临床提供了理论支持。肾脏作为机体的重要脏器之一,通过研究它的急性损伤的机制可以更好的理解MODS的发病机理。
Objective To construct a dog's MODS model by injection of endotoxin leading to sepsis in dogs and the formation of multiple organ failure.By studying the indicators of kidneys,we can further observe the kidney damage of MODS dogs,and better understand the mechanism of kidney damage in MODS dogs,and provide theoretical basis for clinical and experimental research.Methods Fourteen dogs were divided into two groups,including 7 dogs with multiple organ failure models(experimental group)and 7 normal dogs(control group).Learn more about kidney damage by measuring kidney indicators over time.Results According to the experiment,the MODS model dog group(experimental group)compared with the normal dog group(control group),the experimental group canine indicators of renal function urea nitrogen and creatinine began to show a progressive increase 2 hours after the start of the experiment(P<0.05),the urine output of the experimental group was significantly reduced compared with the control group,and the time of polyuria was significantly delayed.The pathological results showed that sepsis would cause damage to the renal tubules,but diffuse necrosis did not occur(P<0.05).Conclusion In the experimental dog,the kidney indicators began to rise 2 hours after the model was successfully established.The kidney did have acute damage.Pathology also proved the kidney damage,but the renal tubules did not undergo diffuse necrosis.Experiments have confirmed that if you want to save the kidney function of experimental dogs,you need to actively search for the cause of kidney damage 2 hours ago,actively treat the primary disease,and provide theoretical support for the clinic.The kidney is one of the important organs of the body,and the pathogenesis of MODS can be better understood by studying the mechanism of its acute injury.
作者
张健
李发鹏
阿不都克里木·阿不都
汤宝鹏
ZHANG Jian;LI Fapeng;ABUDOUKELIMU·Abudou;TANG Baopeng(CCU,Cardiovascular Disease Center,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)
出处
《西部医学》
2024年第9期1260-1264,共5页
Medical Journal of West China
基金
国家自然科学基金项目(82000238)
新疆维吾尔自治区自然科学基金项目(2022D01C237)。
