摘要
以“滇翠8号”黄瓜为试材,通过施加75 mmol·L^(-1)NaCl进行盐胁迫,外源施加不同浓度的EGTA、LaCl_(3)、CaCl_(2)、Ca(NO_(3))_(2),采用温室大棚栽培方法,研究了外源施加Ca^(2+)对盐胁迫下黄瓜幼苗叶片和根系的形态、生理指标的影响,以期为缓解土壤盐分胁迫对黄瓜等蔬菜栽培的危害提供参考依据。结果表明:在盐胁迫下,黄瓜幼苗的株高、根长、茎粗等均受到不同程度的抑制,施加浓度为4.0 mmol·L^(-1)的Ca^(2+)抑制剂(LaCl_(3)和EGTA)对黄瓜幼苗的生长抑制显著,同时降低抗氧化酶系统的活性,增强盐胁迫的危害;而施加5.0 mmol·L^(-1)的CaCl_(2)和Ca(NO_(3))_(2)能够缓解盐胁迫,促进黄瓜幼苗的生长,且显著降低丙二醛和脯氨酸含量,激活并加强抗氧化酶(过氧化物酶、超氧化物歧化酶)的活性。
Taking cucumber‘Diancui 8’as the test material,treatment was carried out by applying 75 mmol·L^(-1) NaCl and exogenous application of different concentrations of EGTA,LaCl_(3),CaCl_(2),Ca(NO_(3))_(2).The effects of exogenous application of Ca^(2+)on the morphology and physiological indicators of cucumber seedling leaves and roots under salt stress were studied,in order to provide reference for alleviating the harm of soil salt stress on cucumber and other vegetable cultivation.The results showed that under salt stress,the plant height,root length,and stem diameter of cucumber seedlings were inhibited to varying degrees.The application of Ca^(2+)inhibitors(LaCl_(3) and EGTA)with a concentration of 4.0 mmol·L^(-1) significantly inhibited the growth of cucumber seedlings,while reducing the activity of antioxidant enzyme systems and increasing the harm of salt stress.Applying 5.0 mmol·L^(-1) of CaCl_(2) and Ca(NO_(3))_(2) could alleviate salt stress,promote the growth of cucumber seedlings,significantly reduce the content of malondialdehyde and proline,activate and strengthen the activity of antioxidant enzyme(peroxidase,superoxide dismutase).
作者
赵文麟
张新梅
梁飞飞
张国平
许俊强
李振林
ZHAO Wenlin;ZHANG Xinmei;LIANG Feifei;ZHANG Guoping;XU Junqiang;LI Zhenlin(Yunnan Province Diantai Characteristic Agricultural Industrialization Engineering Research Center,Yunnan Agricultural University,Kunming,Yunnan 650201;School of Landscape and Horticulture,Yunnan Agricultural University/Yunnan Key Laboratory of Vegetable Biology,Kunming,Yunnan 650201)
出处
《北方园艺》
CAS
北大核心
2024年第16期1-8,共8页
Northern Horticulture
基金
云南省农业联合专项资助项目(202101BD070001-115)
云南省基础研究资助项目(202201AT070502)
云南省重大科技专项资助项目(202402AE090012)
云南省科技人才和平台计划资助项目(202205AF150017,202205AF150021)
云南省教育厅科学研究资助项目(2024Y300)。
