摘要
【目的】椭圆食粉螨Aleuroglyphus ovatus(Troupeau)是自然环境中最常见的储藏物害螨之一,造成了严重的经济损失。本研究旨在探究不同条件紫外线(Ultraviolet,UV)对于椭圆食粉螨的生理影响、卵孵化率和产卵量影响,以求为居家环境及储备粮仓除螨提供理论依据和防治新思路。【方法】使用不同波长紫外线UV-A(365 nm)、UV-B(308 nm)和UV-C(254 nm),功率均为15 W,分别处理各个发育历期(卵、幼螨、前若螨、后若螨、成螨)的椭圆食粉螨,统计其死亡率、卵孵化率、产卵量并对比昆虫卵黄原蛋白(Vitellogenin,Vg)基因表达量,最后使用IBM SPSS Statistics 25.0和GraphPad Prism 9.5.0对数据进行统计分析。【结果】比较3种波长处理组的结果,UV-B的影响最大,照射180 min后,椭圆食粉螨各虫态死亡率均达100%。在时间方面,死亡率与照射时间呈正相关,即在相同距离下,照射时间越长,粉螨的死亡率越高。在紫外线短期辐射后,产卵量(除日产卵量外)和Vg基因表达量均显著提高或存在升高趋势(P<0.05)。UV-C导致的椭圆食粉螨雌成螨生殖力提高最为显著(P<0.05),产卵量增加51.15%。【结论】3种波长的紫外线均对椭圆食粉螨的生长发育有抑制作用,且UV-B对螨的杀灭效果优于UV-A和UV-C。在不同应用场景中,可以使用UV-B辐射灯进行高效除螨。
[Aim]Aleuroglyphus ovatus(Troupeau)is a widespread storage mite that causes serious economic losses.This study aimed to investigate the effect of different ultraviolet(UV)wavelengths on the survival,hatching rate,and fecundity of A.ovatus,providing a theoretical basis and an innovative approach for managing mite infestations in domestic environments and grain storage areas.[Methods]The mortality of A.ovatus at each developmental stage(adult,tritonymph,protonymph,larvae,and egg),egg hatching rate,egg laying capacity,and vitellogenin(Vg)gene expression were measured after treatments with UV-A(365 nm),UV-B(308 nm),and UV-C(254 nm)(15 W).Statistical analysis was carried out using IBM SPSS Statistics 25.0 and GraphPad Prism 9.5.0.[Results]Among the three wavelength treatment groups,UV-B demonstrated the most significant effect on A.ovatus.Following 180 minutes of UV-B irradiation,the mortality rate of A.ovatus reached 100%across all developmental stages.Furthermore,the mortality rate was positively correlated with the irradiation time.Following short-term ultraviolet radiation,there was a significant increase or an increasing trend in the number of eggs laid(in addition to the daily number of eggs)and an increase in the expression level of the Vg gene(P˂0.05)The UV-C treatment group had a significantly higher reproductive capacity(51.15%)compared to the UV-A and UV-B treatment groups(P˂0.05).[Conclusion]All three ultraviolet wavelengths can inhibit the growth and development of A.ovatus.The mortality effect of UV-B was superior to UV-A and UV-C.Therefore,UV-B radiation lamps can serve as an effective method for mite control across various application scenarios.
作者
孙文萱
邹杨
苗罡
张锦楠
郑洪峰
杨远发
邹志文
SUN Wen-Xuan;ZOU Yang;MIAO Gang;ZHANG Jin-Nan;ZHENG Hong-Feng;YANG Yuan-Fa;ZOU Zhi-Wen(School of Life Science,Nanchang University,Nanchang 330031,China;Fu’an Acepow Equipment Co.,Ltd.,Ningde 355017,China;Jiangxi Provincial Key Laboratory of Interdisciplinary Science,Nanchang University,Nanchang 330031,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2024年第3期615-624,共10页
Chinese Journal of Applied Entomology
基金
国家自然科学基金(31860601)
江西省重点研发计划(20212BBF63042,20203BBF63041)
南昌大学自然科学跨学科创新基金(9167-28220007-YB2105)。
