摘要
目的观察烟碱型胆碱受体α5(CHRNA5)在前列腺癌中的表达及对前列腺癌DU145细胞恶性转移的影响。方法前列腺癌组织及癌旁正常前列腺组织取自2022年1月至2023年10月于天津医科大学总医院接受手术治疗的15例前列腺腺癌患者。免疫组织化学实验检测前列腺癌癌组织以及癌旁正常前列腺组织CHRNA5的表达水平;将前列腺癌细胞系DU145细胞分为3个实验组:si CHRNA5组、si NC组以及Yes相关蛋白1/PDZ结合域的转录共刺激因子(YAP1/TAZ)抑制剂组。通过四甲基偶氮唑蓝(MTT)法检测DU145细胞的增殖能力;通过Transwell实验分析DU145细胞的侵袭能力;通过细胞划痕实验分析DU145细胞的迁移能力;通过流式细胞术分析DU145细胞的凋亡率;通过蛋白免疫印迹检测YAP1/TAZ信号通路以及CHRNA5蛋白的表达水平。两组间比较采用独立样本t检验。结果前列腺癌组织CHRNA5的表达水平高于癌旁正常前列腺组织(0.28±0.07比0.89±0.08,t=12.178,P<0.05)。si CHRNA5以及YAP1/TAZ抑制剂组的DU145细胞增殖能力低于si NC组(1.72±0.06比0.83±0.01比0.81±0.05,F=11.369,P<0.05);si CHRNA5以及YAP1/TAZ抑制剂组的DU145细胞侵袭数低于si NC组(139.19±10.76比83.89±9.36比79.93±8.25,F=21.328,P<0.05);si CHRNA5以及YAP1/TAZ抑制剂组的DU145细胞迁移率低于si NC组[(88.65±6.06)%比(55.86±5.33)%比(56.23±3.88)%,F=20.162,P<0.05];si CHRNA5以及YAP1/TAZ抑制剂组的DU145细胞凋亡率高于si NC组[(3.16±0.36)%比(12.62±0.25)%比(13.26±0.17)%,F=13.179,P<0.05];si CHRNA5以及YAP1/TAZ抑制剂组的DU145细胞YAP1、TAZ蛋白水平低于si NC组(0.81±0.12比0.32±0.15比0.28±0.09,F=15.782,P<0.05;0.79±0.12比0.18±0.09比0.22±0.06,F=17.336,P<0.05);pcDNA3.1 CHRNA5组DU145细胞的YAP1、TAZ蛋白表达水平高于pcDNA3.1 NC组(0.32±0.06比0.78±0.03,t=11.569,P<0.05;0.26±0.07比0.72±0.03,t=16.238,P<0.05)。结论CHRNA5在前列腺癌组织中高表达,CHRNA5能够通过激活YAP1/TAZ通路促进前列腺癌DU145细胞
ObjectiveTo investigate the expression of cholinergic receptor nicotinic alpha 5(CHRNA5)in prostate cancer and its effect on the malignant metastasis of prostate cancer DU145 cells.MethodsProstate cancer tissues and adjacent normal prostate tissues were obtained from 15 prostate adenocarcinoma patients who underwent surgery in Tianjin Medical University General Hospital from January,2022 to October,2023.Immunohistochemistry was used to detect the expression level of CHRNA5 in prostate cancer tissues and adjacent normal prostate tissues.DU145 cells were divided into three experimental groups:si CHRNA5 group,si NC group and yes-associated protein 1/Transcriptional coactivator with PDZ-binding motif(YAP1/TAZ)inhibitor group.The proliferation of DU145 cells was detected by methylthiazolyl-tetrazolium(MTT)assay.The invasion ability of DU145 cells was analyzed by Transwell assay.The migration ability of DU145 cells was analyzed by cell scratch test.The apoptosis rate of DU145 cells was analyzed by flow cytometry.The expression levels of YAP1/TAZ signaling pathway and CHRNA5 protein were detected by Western blotting.Comparison between the two groups was done using independent sample t test.ResultsThe expression level of CHRNA5 in prostate cancer tissues was higher than that in adjacent normal prostate tissues(0.28±0.07 vs.0.89±0.08,t=12.178,P<0.05).The proliferation ability of DU145 cells in si CHRNA5 and YAP1/TAZ inhibitor groups was lower than that in si NC group(1.72±0.06 vs.0.83±0.01 vs.0.81±0.05,F=11.369,P<0.05).The invasion number of DU145 cells in the si CHRNA5 and YAP1/TAZ inhibitor groups was less than that in the si NC group(139.19±10.76 vs.83.89±9.36 vs.79.93±8.25,F=21.328,P<0.05).The migration rate of DU145 cells in the si CHRNA5 and YAP1/TAZ inhibitor groups was lower than that in the si NC group[(88.65±6.06)%vs.(55.86±5.33)%vs.(56.23±3.88)%,F=20.162,P<0.05].The apoptosis rate of DU145 cells in the si CHRNA5 and YAP1/TAZ inhibitor groups was higher than that in the si NC group[(3.16±0.36)%vs.(12
作者
樊黎明
张晓方
任亚女
张强
张靓男
Fan Liming;Zhang Xiaofang;Ren Yanv;Zhang Qiang;Zhang Liangnan(Department of Clinical Laboratory,General Hospital of Tianjin Medical University,Tianjin 300052,China;Department of Laboratory,Branch Hospital of Tianjin Third Central Hospital,Tianjin 300250,China)
出处
《中华实验外科杂志》
CAS
2024年第8期1767-1771,共5页
Chinese Journal of Experimental Surgery
基金
国家重点研发计划(2021YFC2009300)。
关键词
烟碱型胆碱受体α5
前列腺癌
转移
Cholinergic receptor nicotinicα5
Prostate cancer
Metastasis
