摘要
本研究旨在探索小黄鱼酶解产物对嗜热链球菌FUA329的增殖作用,并测定不同分子量酶解产物的抗氧化活性。在选择不同酶水解小黄鱼加工副产物的基础上,选用木瓜蛋白酶对小黄鱼副产物进行酶解。以酶解产物对嗜热链球菌的增殖效应为评价指标,采用单因素实验和响应面法优化酶解条件,并对酶解产物的体外抗氧化活性进行测定。结果表明,小黄鱼加工副产物酶解的最佳工艺参数为:酶解时间5 h,料液比1:5.1(w/v),加酶量2.8%。将优化条件下制备的小黄鱼酶解液按分子量大小分为4个片段:>10000 Da,5000~10000 Da,3000~5000 Da,<3000 Da,其中分子量<3000 Da的片段促进嗜热链球菌的增殖能力最强。小黄鱼酶解产物对于DPPH、羟自由基和超氧阴离子自由基的清除率分别达到68.75%、56.23%和30.64%。小黄鱼酶解产物对于嗜热链球菌的增殖有一定的促进效果,同时具有一定的抗氧化能力,有开发为嗜热链球菌氮源的潜力。
To determine the promoting effects of enzyme hydrolysis products of Larimichthys polyactis on the proliferation of Streptococcus thermophilus FUA329.The in vitro antioxidant activity of the enzymatic hydrolysis with different molecular weights were determined.Papain was selected for the enzymatic hydrolysis of its by-products on the basis of selecting different enzymes to hydrolyze the processing by-products of Larimichthys polyactis.Taking the proliferative effect of enzymatic degradation products on Streptococcus thermophilus as an indicator of evaluation.The enzymatic hydrolysis conditions were optimized through single factor and response surface methodology.The in vitro antioxidant activity of the hydrolysis products of the by-products of Larimichthys polyactis(HPBL)were determined.The results showed that the best enzymatic hydrolysis conditions included the hydrolysis time,material-liquid ratio and enzyme loading were 5 h,1:5.1(w/v)and 2.8%(w/v),respectively.The HPBL prepared under the optimized conditions was separated by ultrafiltration into four fragments,according to the molecular weight:>10000 Da,5000~10000 Da,3000~5000 Da,<3000 Da,among which the fragment with molecular weight of less than 3000 Da promoted the proliferation of Streptococcus thermophilus significantly.The scavenging rate of HPBL for DPPH,hydroxyl radicals and superoxide anion radicals reached 68.75%,56.23%and 30.64%,respectively.The enzyme hydrolysis products of Larimichthys polyactis significantly promoted the proliferation of Streptococcus thermophilus FUA329 and showed antioxidant activity.HPBL would have a certain promoting effect on the proliferation of Streptococcus thermophilus FUA329,and a certain antioxidant capacity,which would have the potential to be developed as a nitrogen source for Streptococcus thermophilus.
作者
陆笑月
华梓延
吴晴
吴浩
刘姝
杨光
侯晓月
房耀维
LU Xiaoyue;HUA Ziyan;WU Qing;WU Hao;LIU Shu;YANG Guang;HOU Xiaoyue;FANG Yaowei(School of Ocean Food and Biological Engineering,Jiangsu Ocean University,Lianyungang 222005,China)
出处
《食品工业科技》
CAS
北大核心
2024年第18期138-145,共8页
Science and Technology of Food Industry
基金
国家自然科学基金青年科学基金项目(32102270)
江苏省高等学校自然科学研究重大项目(20KJA55001)
江苏省“333高层次人才培养工程”项目(BRA2019243)。
关键词
小黄鱼
嗜热链球菌
促进增殖
蛋白酶水解
酶解工艺优化
抗氧化活性
Larimichthys polyactis
Streptococcus thermophilus
promotion of proliferation
protease hydrolysis
enzymatic process optimization
antioxidant activity
