摘要
目的 明确腹腔感染耐药菌小鼠的半数致死浓度(LC50)和绝对致死浓度(LC100),探讨R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠的抗炎作用。方法 随机将小鼠分为正常对照组(NC组)、耐药菌株感染模型组(Model组)、R-(+)-长叶薄荷酮组(PU组,20 mg/kg)、头孢克肟组(CFM组,26 mg/kg),每组10只;除NC组外,其他试验组进行干预,连续3 d, 1次/d, Model组按照20 ml/kg经口灌胃食用油,PU组、CFM组分别给予等体积的R-(+)-长叶薄荷酮、头孢克肟,第3天给药后1 h,腹腔注射耐药大肠埃希菌(10 ml/kg),观察24、48 h后收集小鼠眼眶血、腹腔冲洗液、腹膜组织;采用全自动细胞分析仪进行血液、腹腔冲洗液的白细胞计数;瑞氏-吉姆萨染色观察血细胞形态变化;苏木精-伊红(HE)染色法观察腹腔组织病理形态变化。结果 腹腔感染耐药菌小鼠的LC50和LC100分别是1.50×10~8 CFU/ml、2.25×10~8 CFU/ml;与NC组相比,Model组小鼠腹腔内存在弥漫性炎症,腹腔充血、水肿;而PU组、CFM组小鼠腹腔内炎症、充血、水肿程度较Model组不明显;眼眶血白细胞计数表明PU组较NC组有所降低,较Model组、CFM组无统计学差异;腹腔冲洗液白细胞计数PU组低于Model组,较NC组增高,较CFM组无统计学差异,表明R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠具有抗炎作用(P<0.05);HE结果显示,PU组、CFM组腹膜组织结构完整,间皮细胞排列整齐,腹膜组织间质较NC组可见少量淋巴细胞浸润,相对于Model组腹膜组织结构更加完整,淋巴细胞浸润较少,表明R-(+)-长叶薄荷酮对腹腔耐药菌的感染具有显著的抗感染保护作用。结论 R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠具有一定抗炎作用,为R-(+)-长叶薄荷酮抗感染药理作用机制的探索提供一定的研究基础。
OBJECTIVE To determine the median lethal concentration(LC5O)and absolute lethal concentration(LC1oo)of drug-resistant bacteria in mice infected intraperitoneally,and to investigate the anti-inflammatory effect of R-(+)-pulegone on mice infected with drug-resistant bacteria in the abdominal cavity.METHODS Mice were randomly divided into the normal control group(NC group),the drug-resistant strain infection model group(Model group),the R-(+)-pulegone group(PU group,20 mg/kg),and the cefixime group(CFM group,26 mg/kg),with 1o mice in each group.Except for the NC group,other test groups were intervened for three consecutive days,once a day.The Model group was given 20 ml/kg edible oil orally,and the PU group and CFM group were given an equal volume of R-(+)-pulegone and cefixime,respectively.On the third day,one hour after administration,drug-resistant Escherichia coli(10 ml/kg)was intraperitoneally injected.After observation for 24 and 48 hours,orbital blood,peritoneal lavage fluid,and peritoneal tissue of the mice were collected.A fully automated cell analyzer was used to count white blood cells in blood and abdominal cavity rinse fluid.Morphological changes of blood cells was observed by using Wright-Giemsa staining assay,and pathological changes in the abdominal tissue was detected by hematoxylin-eosin(HE)staining.RESULTS LC50 and LC100 of drug-resistant bacteria intraperitoneally inoculated in the mice were 1.50×10^(8) CFU/ml and 2.25×10^(8) CFU/ml,respectively.Compared with the NC group,there were diffuse inflammation,hyperemia and edema in the Model group,while the pathological changes in the PU group and CFM Group were not obvious.The WBC count in the PU group was significantly lower than that in the NC group,but not significantly different from that in the CFM Group and Model group.The WBC count in peritoneal lavage fluid in the PU group was lower than that in the Model group,while it was higher than that in the NC group,but not significantly higher than the CFM Group,which indicated that R-(+)-pulegone ha
作者
宫海燕
杨珊珊
王晓娟
郑琳川
季兴磊
熊文娟
GONG Hai-yan;YANG Shan-shan;WANG Xiao-juan;ZHENG Lin-chuan;JI Xing-lei;XIONG Wen-juan(The First Dongguan Affiliated Hospital,Guangdong Medical University,Dongguan,Guangdong 523710,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2024年第16期2405-2410,共6页
Chinese Journal of Nosocomiology
基金
国家自然科学基金资助项目(82060748)。
