摘要
目的探讨白介素33(interleukin-33,IL-33)和白介素13(interleukin-13,IL-13)在宫颈癌患者中的表达情况及其预测预后的价值。方法选取2020年1月至2022年1月收治于新疆医科大学第一附属医院的40例宫颈癌患者的宫颈组织,同时选取30例子宫肌瘤经宫颈癌筛查为阴性的患者的宫颈组织为对照,使用免疫组织化学(IHC)和实时荧光定量PCR(qRT-PCR)技术比较两组宫颈组织中IL-33和IL-13的表达情况;另外收集99例宫颈癌患者(宫颈癌组)及50例正常健康个体(对照组)的血清样本,使用酶联免疫吸附法(ELISA)检测两组血清样本中IL-33和IL-13的表达情况,进一步通过利用String数据库和Person相关性分析评估IL-33和IL-13的关系。接下来根据提供宫颈癌组织的40例宫颈癌患者的15个月生存结局将其分为生存组(22例)和死亡组(18例),再根据提供血清样本的99例宫颈癌患者的18个月生存结局将其分为生存组(59例)和死亡组(40例),使用Kaplan-Meier生存曲线和log-Rank检验方法进行生存分析;最后比较两组数据并进行二元Logistic回归分析和受试者工作特征(ROC)曲线的绘制。结果(1)免疫组化结果显示,宫颈癌组织中IL-33和IL-13呈阳性表达,其MOD值高于正常宫颈组织(P<0.05);qRT-PCR结果显示,IL-33和IL-13的相对mRNA表达显著高于对照组(P<0.001)。(2)ELISA结果显示,宫颈癌患者血清中的IL-33和IL-13明显高于正常健康个体(P<0.001)。String数据库显示IL-33和IL-13有相互作用;Person相关性分析显示IL-33与IL-13呈正相关(P<0.05,r=0.6382)。(3)二元Logistic回归分析显示肿块≥4 cm、PRL、IL-33和IL-13增高是宫颈癌患者死亡的独立危险因素(均P<0.05)。(4)ROC分析显示IL-33和IL-13预测宫颈癌患者生存结局的曲线下面积(AUC)分别为0.842、0.899;最佳截断值为31.385 pg/mL、10.772 pg/mL。结论无论是宫颈癌患者组织中还是血清中,IL-33和IL-13均呈现高表达,且其有助于预测宫颈癌患者预后,�
Objective To investigate the expression of intereukin-33(IL-33)and intereukin-13(IL-13)in patients with cervical cancer and their prognostic value.Methods Cervical tissues from 40 cervical cancer patients admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2020 to January 2022 were selected,while cervical tissues from 30 patients with cervical cancer screening negative for cervical leiomyomas were selected as controls,and the expression of IL-33 and IL-13 in cervical tissues of the two groups were compared using immunohistochemistry(IHC)and real-time fluorescence quantitative PCR(qRT-PCR)techniques.In addition,serum samples were collected from 99 patients with cervical cancer(cervical cancer group)and 50 normal healthy individuals(control group),and the expression of IL-33 and IL-13 in the two groups was detected using enzyme-linked immunosorbent assay(ELISA),and the relationship between IL-33 and IL-13 was evaluated by using the String database and Person correlation analysis.Subsequently,40 cervical cancer patients who submitted cervical cancer tissues were categorized into survival group(22 cases)and death group(18 cases)according to their 15-month survival results.Additionally,99 cervical cancer patients who provided serum samples were segregated into survival group(59 cases)and death group(40 cases)based on their 15-month survival outcomes.Survival analysis was conducted using Kaplan-Meier survival curve and log-Rank test method.The data of the two groups were compared and the binary Logistic regression and receiver operating characteristic curve(ROC)were plotted.Results(1)The immunohistochemical results showed that the expression of IL-33 and IL-13 in cervical cancer tissues was positive,and the MOD value was significantly higher than that in normal cervical tissues(P<0.05).qRT-PCR results showed that the relative mRNA expression of IL-33 and IL-13 was significantly higher than that of the control group(P<0.001).(2)ELISA results showed that serum IL-33 and IL-13 in patients
作者
王碧辉
朱玥洁
张玉莲
赵丽媛
吴雨峰
陈志芳
Wang Bihui;Zhu Yueje;Zhang Yulian;Zhao Liyuan;Wu Yufeng;Chen Zhifang(Gynecology Center,The First Affiliated Hospital of Xinjiang Medical University;State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia;Reproductive Center,The First Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 83001l,P.R.China)
出处
《中国计划生育和妇产科》
2024年第8期95-100,F0003,共7页
Chinese Journal of Family Planning & Gynecotokology
基金
国家自然科学基金资助项目(项目编号:82160545)
省部共建中亚高发病成因与防治国家重点实验室开放课题项目(项目编号:SKL-HIDCA-2022-5)。
