摘要
目的研究不同检测方法在梅毒特异性抗体筛查诊断中的价值。方法选取2020年1月-2023年4月我院诊断的60例梅毒患者为研究对象,均行电化学发光免疫法(ECLIA)、化学发光免疫分析(CLIA)、酶联免疫吸附实验(ELISA)检测梅毒特异性抗体,并以快速梅毒螺旋体抗体胶体金法(金标法)作为标准,比较不同检测方法检测结果、诊断效能(灵敏度、特异度、准确率、阳性预测值、阴性预测值)、与金标法检测结果一致性。结果ECLIA、CLIA法S/CO<1时,所有标本经金标法复检均为阴性;ELISA法S/CO<1时,其中2例样本经金标法检测为阳性;ECLIA法S/CO≥5时、CLIA法S/CO≥7时,ELISA法S/CO≥7时,金标法检测阳性率均为100.00%;ECLIA、CLIA、ELISA法检测梅毒特异性抗体灵敏度、特异度、准确率、阳性预测值、阴性预测值比较,差异无统计学意义(P>0.05);ECLIA、CLIA、ELISA法检测梅毒特异性抗体检验结果与金标法检测结果一致性比较,差异无统计学意义(P>0.05);ROC曲线分析显示,ECLIA、CLIA法诊断梅毒的AUC面积比较,差异无统计学意义(P>0.05),而CLIA、ECLIA法诊断梅毒的AUC面积均大于ELISA比较,差异有统计学意义(P<0.05)。结论当ECLIA、CLIA、ELISA法对梅毒进行筛查时S/CO分别小于5、7、7,需采用金标法进行验证,以减少误诊情况。但相对比较,ECLIA法、CLIA法诊断效能高于ELISA法。
Objective To study the value of different detection methods in the screening and diagnosis of syphilis-specific antibodies.Methods A total of 60 patients with syphilis diagnosed in our hospital from January 2020 to April 2023 were selected as the research objects.Electrochemiluminescence immunoassay(ECLIA),chemiluminescence immunoassay(CLIA)and enzyme-linked immunosorbent assay(ELISA)were used to detect syphilis-specific antibodies.The colloidal gold method of rapid treponema pallidum antibody(gold standard method)was used as the standard to compare the results of different detection methods,diagnostic efficacy(sensitivity,specificity,accuracy,positive predictive value,negative predictive value),and consistency with the results of gold standard method.Results When ECLIA and CLIA S/CO<1,all specimens were negative by gold standard method.When S/CO<1 by ELISA,2 samples were positive by gold standard method.When S/CO≥5 by ECLIA,S/CO≥7 by CLIA and S/CO≥7 by ELISA,the positive rate ofgold standard method was 100.00%.There was no significant difference in the sensitivity,specificity,accuracy,positive predictive value and negative predictive value of ECLIA,CLIA and ELISA in the detection of syphilis specific antibody(P>0.05).There was no significant difference in the consistency between the results of syphilis specific antibody detection by ECLIA,CLIA,ELISA and gold standard method(P>0.05).ROC curve analysis showed that there was no significant difference in AUC area between ECLIA and CLIA in the diagnosis of syphilis(P>0.05),while the AUC area of CLIA and ECLIA in the diagnosis of syphilis was greater than that of ELISA(P<0.05).Conclusion When ECLIA,CLIA and ELISA are used to screen syphilis,S/CO is less than 5,7 and 7,respectively,gold standard method should be used for verification to reduce misdiagnosis.However,the diagnostic efficiency of ECLIA and CLIA was higher than that of ELISA.
作者
冯林冬
FENG Lin-dong(Laboratory Department of Jiujiang Chaisang District People's Hospital,Jiujiang 332105,Jiangxi,China)
出处
《医学信息》
2024年第17期146-149,共4页
Journal of Medical Information
关键词
梅毒特异性抗体
梅毒
筛查作用
诊断效能
Syphilis specific antibody
Syphilis
Screening function
Diagnostic efficacy
