摘要
为探明吉祥草[Reineckea carnea(Andrews.)Kunth]种子的萌发与贮藏特性,通过改变温度及光照条件,并进行硅胶快速脱水处理和种子萌发测试,对新鲜成熟的吉祥草种子萌发特性与脱水耐性进行研究。结果表明,新鲜成熟的吉祥草种子无休眠特性,最适发芽温度为20~25℃恒温;交替光照处理时的发芽率显著高于黑暗处理,应为需光种子,然而交替光照处理时的发芽速率显著慢于黑暗处理,符合耐阴的生态特性;新鲜成熟的吉祥草种子较不耐脱水,当含水量从初始的(19.8±0.1)%分别脱水至(9.0±0.2)%和(6.0±0.0)%时,发芽率从(88.0±2.2)%分别降至(59.0±6.4)%和(15.5±1.5)%,判断其应为顽拗性种子;另外,相较于发芽率、发芽势和平均发芽日数,发芽指数的变化对于种子脱水处理的响应最敏感,可以作为检测种子脱水耐性的首选指标。该研究能够促进吉祥草的应用、种子的长期保存与种质创新。
In order to investigate the germination and storage characteristics of Reineckea carnea(Andrews.)Kunth seeds,a preliminary study was carried out on the germination and dehydration tolerance of freshly matured R.carnea(Andrews.)Kunth seeds through changes in temperature,light,rapid dehydration treatment with silica gel and germination test.The results showed that the fresh mature R.carnea(Andrews.)Kunth seeds were non-dormant,and their optimal germination temperature was a constant temperature of 20~25℃.The germination percentage under the alternating light treatment was significantly higher than that under the dark treatment,which should be a photoblastic seed,yet the germination rate under the alternating light treatment was significantly slower than that under the dark treatment,which was implicitly consistent with their shade-tolerant ecological characteristics.The fresh mature R.carnea(Andrews.)Kunth seeds were less resistant to dehydration and were recalcitrant in that the germination percentage decreased from(88.0±2.2)%to(59.0±6.4)%and(15.5±1.5)%when the moisture content decreased from the initial(19.8±0.1)%to(9.0±0.2)%and(6.0±0.0)%,respectively.Compared to germination percentage,germination potential,and average number of days to germination,the change in germination index was the most sensitive in response to seed dehydration treatments,so it could be used as a preferred index when testing seed dehydration tolerance.This study will improve the application,long-term seed conservation and germplasm innovation of R.carnea(Andrews.)Kunth seeds.
作者
申雪洋
刘相泉
乐靖宇
邓志军
SHEN Xueyang;LIU Xiangquan;LE Jingyu;DENG Zhijun(Hubei Key Laboratory of Biologic Resources Protection and Utilization,Enshi 445000,China;Research Center for Germplasm Engineering of Characteristic Plant Resources in Enshi Prefecture,Hubei Minzu University,Enshi 445000,China)
出处
《湖北民族大学学报(自然科学版)》
CAS
2024年第3期301-306,共6页
Journal of Hubei Minzu University:Natural Science Edition
基金
国家自然科学基金项目(31860073)
生物资源保护与利用湖北省重点实验室开放基金项目(KYPT012313)。
关键词
吉祥草
交替光照
种子休眠
发芽指数
脱水处理
顽拗性种子
Reineckea carnea(Andrews.)Kunth
alternating light
seed dormancy
germination index
dehydration treatment
recalcitrant seed