摘要
为了探究不同产地枳壳中稀土的分布规律、元素含量及内在联系并绘制枳壳稀土元素指纹图谱作为鉴定枳壳中药材的参考依据,本文运用碰撞反应池(KED)模式和在线内标法,采用微波消解-ICPMS法同时测定枳壳中的La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Ho、Er、Tm、Lu、Yb、Y等15种稀土元素。各元素相关系数R2在0.996~1.000,精密度RSD为1.4%~5.1%,说明线性关系良好,样品回收率为80%~106%,RSD为0.3%~5.6%,所建立的方法可用于对枳壳中稀土元素的快速准确检测。检测结果显示,枳壳样品中稀土元素含量丰富,其中Y、La、Ce、Nd元素含量相对较高,Y含量最高达到了255.9μg/kg,La含量最高可达365.3μg/kg,Ce含量最高达到了245.0μg/kg;然而Lu含量在不同产地枳壳中均未检出。以各产地稀土元素含量的平均值绘制枳壳稀土元素标准指纹图谱,作为鉴别枳壳的参考依据。本文采用Pearson相关性分析了枳壳中13种稀土元素(Lu未检出,Tm仅少量样品检出),结果表明稀土元素间均呈正相关,其中Ce与Y、Dy、Ho、Er、Yb呈显著正相关(^(*)p<0.05),其他元素之间呈极显著正相关(^(**)p<0.01)。本文采用TBtools软件进行聚类热图分析,分析结果表明,江西的(除S5)聚为一类,其余的聚为一类。本文采用Origin 2022进行主成分分析,与聚类热图分析结果一致,全国各产地枳壳稀土元素指纹图谱具有较高的一致性和特征性,可为枳壳资源的真假鉴别工作提供研究思路,为其质量安全提供基础数据。
In order to explore the distribution law,element content and internal relationship of rare earth elements in Fructus Aurantii from different producing areas,and to draw the fingerprint of rare earth elements in Fructus Aurantii,as a reference for the identification of Fructus Aurantii medicinal materials.A method for simultaneous determination of La,Ce,Pr,Nd,Sm,Eu,Gd,Tb,Dy,Ho,Er,Tm,Lu,Yb and Y in Fructus Aurantii by microwave digestion-ICP-MS with collision reaction cell(KED)mode and on-line internal standard method was developed.The correlation coefficient R2 of each element was 0.996~1.000,and the precision RSD was 1.4%~5.1%,indicating a good linear relationship.The sample recovery rate was 80%~106%,and the RSD was 0.3%~5.6%.The established method can be used for rapid and accurate detection of rare earth elements in Aurantii Fructus.The content of rare earth elements in Aurantii Fructus samples is rich,among which the contents of Y,La,Ce and Nd are relatively high.The content of Y is up to 255.9μg/kg,the content of La is up to 365.3μg/kg,and the content of Ce is up to 245.0μg/kg.However,Lu was not detected.The standard fingerprint of rare earth elements in Fructus Aurantii was drawn based on the average content of rare earth elements in each producing area,which was used as a reference for identifying Fructus Aurantii.Pearson correlation analysis was used to analyze 13 rare earth elements in Aurantii Fructus(Lu was not detected,and Tm was only detected in a small number of samples).The results showed that there was a positive correlation between rare earth elements,among which Ce was significantly positively correlated with Y,Dy,Ho,Er and Yb(^(*)p<0.05),and other elements were significantly positively correlated(^(**)p<0.01).The clustering heat map analysis was carried out by TBtools software.The analysis results showed that Jiangxi was clustered into one category(except S5),and the rest were clustered into one category.The principal component analysis was carried out by Origin 2022,which was consistent wit
作者
洪家顺
王裕成
刘威
宁知贵
HONG Jiashun;WANG Yucheng;LIU Wei;NING Zhigui(Evaluation and Research Center of Daodi Herbs of Jiangxi Province,Nanchang Jiangxi 330029,China)
出处
《江西化工》
CAS
2024年第4期20-25,共6页
Jiangxi Chemical Industry
基金
赣江新区科技计划项目(赣新管创发(2020)26号)。
关键词
枳壳
电感耦合等离子体质谱法
稀土元素
主成分分析
稀土元素指纹图谱
fructus aurantii
inductively coupled plasma mass spectrometry
rare earth element
principal component analysis
rare earth element fingerprint
